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Immunofluorescent staining of human cell line U-2 OS shows localization to nucleoli & microtubules.
Antibody dilution
1:50
Literature conformity
The subcellular location is supported by literature.
IMMUNOHISTOCHEMISTRY
Antibody HPA023081
Antibody HPA023103
Antibody HPA024795
Standard validation
Uncertain
Uncertain
Uncertain
Figure description
Immunohistochemical staining of human testis shows moderate nuclear and cytoplasmic positivity in seminiferous ducts.
Immunohistochemical staining of human testis shows moderate cytoplasmic and nuclear positivity in seminiferous ducts.
Immunohistochemical staining of human testis shows moderate cytoplasmic positivity in seminiferous ducts.
Expression
RNA: detected in 1 tissues Protein: detected in 32 cell types
RNA: detected in 1 tissues Protein: detected in 75 cell types
RNA: detected in 1 tissues Protein: detected in 68 cell types
Retrieval
HIER pH6
HIER pH6
HIER pH6
Antibody dilution
1:200
1:100
1:50
Literature conformity
Partly consistent with extensive gene/protein characterization data.
Partly consistent with extensive gene/protein characterization data.
Partly consistent with extensive gene/protein characterization data.
RNA consistency
Mainly not consistent with RNA expression data.
Mainly not consistent with RNA expression data.
Mainly not consistent with RNA expression data.
WESTERN BLOT
Antibody HPA023081
Antibody HPA023103
Antibody HPA024795
Standard validation
Uncertain
Analysis performed using a standard panel of samples. Current setup is not applicable due to low RNA count.
Supported
Band of predicted size in kDa (+/-20%) with additional bands present.
Supported
Single band corresponding to the predicted size in kDa (+/-20%).
Figure description
Lane 1: Marker [kDa] 250, 130, 95, 72, 55, 36, 28, 17, 10 Lane 2: Negative control (vector only transfected HEK293T lysate) Lane 3: Over-expression Lysate (Co-expressed with a C-terminal myc-DDK tag (~3.1 kDa) in mammalian HEK293T cells, LY410047)
Lane 1: Marker [kDa] 250, 130, 95, 72, 55, 36, 28, 17, 10 Lane 2: Negative control (vector only transfected HEK293T lysate) Lane 3: Over-expression Lysate (Co-expressed with a C-terminal myc-DDK tag (~3.1 kDa) in mammalian HEK293T cells, LY410047)
Target mass (kDa)
76.3
76.3
76.3
Antibody dilution
1:250
1:250
1:250
PROTEIN ARRAY
Antibody HPA023081
Antibody HPA023103
Antibody HPA024795
Standard validation
Supported
Pass with single peak corresponding to interaction only with its own antigen.
Supported
Pass with single peak corresponding to interaction only with its own antigen.
Supported
Pass with single peak corresponding to interaction only with its own antigen.
Figure description
Antibody specificity analysis with protein arrays. Predicted and matching interactions are shown in green.
Antibody specificity analysis with protein arrays. Predicted and matching interactions are shown in green.
Antibody specificity analysis with protein arrays. Predicted and matching interactions are shown in green.
