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NPC1

ANTIBODY INFORMATION
 
 

Antibody HPA026618

  

Antibody CAB070132

  

Provider

 Atlas Antibodies
Sigma-Aldrich
   Novus Biologicals
  

Product name

 HPA026618   NB400-148  

Host species

 Rabbit   Rabbit  

Clonality

 pAb   msAb  

Purity

 Affinity purified using the PrEST-antigen as affinity ligand   Affinity  

Other gene match

    

Released in version

 5   13  

References

    

VALIDATION SUMMARY
 
 

Antibody HPA026618

  

Antibody CAB070132

  
Immunohistochemistry

Approved   Approved  
Western blot

Uncertain   Supported  
Protein array

Approved    

IMMUNOHISTOCHEMISTRY
 
 

Antibody HPA026618

  

Antibody CAB070132

  

Standard validation

 Approved   Approved  

   

Figure description

 Immunohistochemical staining of human gall bladder shows strong cytoplasmic positivity in glandular cells.   Immunohistochemical staining of human kidney shows strong cytoplasmic positivity in renal tubules.  

Expression

 RNA: detected in 37 tissues
Protein: detected in 48 cell types		   RNA: detected in 37 tissues
Protein: detected in 74 cell types		  

Retrieval

 HIER pH6   HIER pH6  

Antibody dilution

 1:50   1:950  

Literature conformity

 Partly consistent with extensive gene/protein characterization data.   Partly consistent with extensive gene/protein characterization data.  

RNA consistency

 Mainly consistent with RNA expression data.   Mainly consistent with RNA expression data.  

WESTERN BLOT
 
 

Antibody HPA026618

  

Antibody CAB070132

  

Standard validation

 Uncertain

Analysis performed using a standard panel of samples.
Only bands not corresponding to the predicted size.		   Supported

Analysis performed using a standard panel of samples.
Band of predicted size in kDa (+/-20%) with additional bands present.		  

   

Figure description

   Lane 1: Marker [kDa] 250, 130, 100, 70, 55, 35, 25, 15, 10
Lane 2: RT4
Lane 3: U-251 MG
Lane 4: Human Plasma
Lane 5: Liver
Lane 6: Tonsil		  

Target mass (kDa)

 142.2, 108.1   142.2, 108.1, 19.7, 19.2, 6.5, 4.6  

Antibody dilution

 1:250   1:500  

PROTEIN ARRAY
 
 

Antibody HPA026618

  

Antibody CAB070132

  

Standard validation

 Approved

Pass with quality comment low specificity (binding to 1-2 PrESTs >15% and <40%).		    

   

Figure description

 Antibody specificity analysis with protein arrays. Predicted and matching interactions are shown in green.    

Antibody dilution

 1:3000    

ANTIGEN INFORMATION
 
 

Antibody HPA026618

  

Antibody CAB070132

  

Antigen

 Recombinant protein fragment   Synthetic peptide  

Length (aa)

 122    

Antigen sequence

 
VLNKVDIGLDQSLSMPDDSYMVDYFKSISQYLHAGPPVYFVLEEGHDYTS
SKGQNMVCGGMGCNNDSLVQQIFNAAQLDNYTRIGFAPSSWIDDYFDWVK
PQSSCCRVDNITDQFCNASVVD
   

Matching transcripts

 NPC1-001 - ENSP00000269228 [100%]
NPC1-003 - ENSP00000467636 [100%]
   

Other gene match

    
ANTIGEN VIEW
NPC1-001
NPC1-003
 

Contact

The Project

The Human Protein Atlas

The Human Protein Atlas project is funded
by the Knut & Alice Wallenberg foundation.