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Immunofluorescent staining of human cell line U-251 MG shows localization to nucleus & vesicles.
Antibody dilution
1:50
Literature conformity
The subcellular location is supported by literature.
IMMUNOHISTOCHEMISTRY
Antibody HPA021573
Antibody HPA023012
Standard validation
Approved
Approved
Figure description
Immunohistochemical staining of human vagina shows distinct cytoplasmic and nuclear positivity in squamous epithelial cells.
Immunohistochemical staining of human stomach shows strong cytoplasmic positivity in parietal cells.
Expression
RNA: detected in 14 tissues Protein: detected in 46 cell types
RNA: detected in 14 tissues Protein: detected in 60 cell types
Retrieval
HIER pH6
HIER pH6
Antibody dilution
1:25
1:75
Literature conformity
Partly consistent with extensive gene/protein characterization data.
Partly consistent with extensive gene/protein characterization data.
RNA consistency
Mainly consistent with RNA expression data.
Mainly not consistent with RNA expression data.
WESTERN BLOT
Antibody HPA021573
Antibody HPA023012
Standard validation
Uncertain
Analysis performed using a standard panel of samples. Only bands not corresponding to the predicted size.
Uncertain
Analysis performed using a standard panel of samples. Single band differing more than +/-20% from predicted size in kDa and not supported by experimental and/or bioinformatic data.
Figure description
Lane 1: Marker [kDa] 230, 130, 95, 72, 56, 36, 28, 17, 11 Lane 2: RT4 Lane 3: U-251 MG Lane 4: Human Plasma Lane 5: Liver Lane 6: Tonsil
Target mass (kDa)
77.4, 33.5
77.4
Antibody dilution
1:250
1:250
PROTEIN ARRAY
Antibody HPA021573
Antibody HPA023012
Standard validation
Approved
Pass with quality comment low specificity (binding to 1-2 PrESTs >15% and <40%).
Supported
Pass with single peak corresponding to interaction only with its own antigen.
Figure description
Antibody specificity analysis with protein arrays. Predicted and matching interactions are shown in green.
Antibody specificity analysis with protein arrays. Predicted and matching interactions are shown in green.
Antibody dilution
1:500
1:3000
ANTIGEN INFORMATION
Antibody HPA021573
Antibody HPA023012
Antigen
Recombinant protein fragment
Recombinant protein fragment
Length (aa)
101
115
Antigen sequence
ACAVDSSEEQKQTAAVALCQLAAYSPRNIRVGDGDAAAPEPACRQDTPTL
SSMESQEAQCDLRPKGQKRTSLRDAGKSQQGAKKAKLQDTARVFTLRRRA
R
