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Immunofluorescent staining of human cell line U-251 MG shows localization to plasma membrane.
Immunofluorescent staining of human cell line A-431 shows localization to plasma membrane.
Antibody dilution
1:30
1:67
Literature conformity
The subcellular location is supported by literature.
The subcellular location is supported by literature.
IMMUNOHISTOCHEMISTRY
Antibody HPA001060
Antibody HPA001338
Antibody HPA001383
Antibody CAB000043
Antibody CAB020416
Antibody CAB062555
Standard validation
Approved
Approved
Approved
Approved
Figure description
Immunohistochemical staining of human urinary bladder shows moderate membranous and cytoplasmic positivity in urothelial cells.
Immunohistochemical staining of human rectum shows weak cytoplasmic positivity in glandular cells.
Immunohistochemical staining of human urinary bladder shows moderate cytoplasmic and membranous positivity in urothelial cells.
Immunohistochemical staining of human urinary bladder shows positivity in urothelial cells.
Expression
RNA: detected in 37 tissues Protein: detected in 57 cell types
RNA: detected in 37 tissues Protein: detected in 18 cell types
RNA: detected in 37 tissues Protein: detected in 30 cell types
RNA: detected in 37 tissues Protein: detected in 22 cell types
Retrieval
HIER pH6
HIER pH9
HIER pH6
HIER pH6
Antibody dilution
1:100
1:1500
1:50
1:80
Literature conformity
Consistent with extensive gene/protein characterization data.
Partly consistent with extensive gene/protein characterization data.
Consistent with extensive gene/protein characterization data.
Partly consistent with extensive gene/protein characterization data.
RNA consistency
Mainly consistent with RNA expression data.
Mainly consistent with RNA expression data.
Mainly consistent with RNA expression data.
Mainly consistent with RNA expression data.
WESTERN BLOT
Antibody HPA001060
Antibody HPA001338
Antibody HPA001383
Antibody CAB000043
Antibody CAB020416
Antibody CAB062555
Standard validation
Uncertain
Analysis performed using a standard panel of samples. Only bands not corresponding to the predicted size.
Uncertain
Analysis performed using a standard panel of samples. Weak band of predicted size but with additional bands of higher intensity also present.
Supported
Analysis performed using a standard panel of samples. Band of predicted size in kDa (+/-20%) with additional bands present.
Uncertain
Analysis performed using a standard panel of samples. Single band larger than predicted size in kDa (+20%) but partly supported by experimental and/or bioinformatic data.
Uncertain
Analysis performed using a standard panel of samples. Only bands not corresponding to the predicted size.
Uncertain
Analysis performed using a standard panel of samples. No bands detected.
Figure description
Lane 1: Marker [kDa] 206, 113, 82, 49, 32, 26, 17.8 Lane 2: RT4 Lane 3: U-251 MG Lane 4: A-431 Lane 5: Liver Lane 6: Tonsil
Lane 1: Marker [kDa] 250, 130, 95, 72, 55, 36, 28, 17, 11 Lane 2: RT4 Lane 3: U-251 MG Lane 4: Human Plasma Lane 5: Liver Lane 6: Tonsil
Lane 1: Marker [kDa] 250, 130, 100, 70, 55, 35, 25, 15, 10 Lane 2: RT4 Lane 3: U-251 MG Lane 4: Human Plasma Lane 5: Liver Lane 6: Tonsil
