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PRDM15

ANTIBODY INFORMATION
 
 

Antibody HPA024268

  

Antibody HPA029255

  

Antibody HPA029256

  

Provider

 Atlas Antibodies
Sigma-Aldrich
   Atlas Antibodies
Sigma-Aldrich
   Atlas Antibodies
Sigma-Aldrich
  

Product name

 HPA024268   HPA029255   HPA029256  

Host species

 Rabbit   Rabbit   Rabbit  

Clonality

 pAb   pAb   pAb  

Purity

 Affinity purified using the PrEST-antigen as affinity ligand   Affinity purified using the PrEST-antigen as affinity ligand   Affinity purified using the PrEST-antigen as affinity ligand  

Other gene match

      

Released in version

 5   14   14  

References

      

VALIDATION SUMMARY
 
 

Antibody HPA024268

  

Antibody HPA029255

  

Antibody HPA029256

  
Immunocytochemistry

 

Validated
  

Validated
  
Immunohistochemistry

Uncertain      
Western blot

Uncertain   Uncertain   Uncertain  
Protein array

Supported   Supported   Supported  

IMMUNOCYTOCHEMISTRY
 
 

Antibody HPA024268

  

Antibody HPA029255

  

Antibody HPA029256

  

Formal validation: Independent

  
Validated

Antibody staining overlaps with antibody HPA029256.		  
Validated

Antibody staining overlaps with antibody HPA029255.		  
 

Standard validation

   Supported   Supported  

     

Figure description

   Immunofluorescent staining of human cell line U-2 OS shows localization to nucleoplasm & nuclear bodies.   Immunofluorescent staining of human cell line U-2 OS shows localization to nuclear bodies.  

Antibody dilution

   1:200   1:110  

Literature conformity

   The subcellular location is supported by literature.   The subcellular location is supported by literature.  

IMMUNOHISTOCHEMISTRY
 
 

Antibody HPA024268

  

Antibody HPA029255

  

Antibody HPA029256

  

Standard validation

 Uncertain      

     

Figure description

 Immunohistochemical staining of human skeletal muscle shows strong cytoplasmic positivity.      

Expression

 RNA: detected in 32 tissues
Protein: detected in 79 cell types		      

Retrieval

 HIER pH6      

Antibody dilution

 1:25      

Literature conformity

 No avaliable gene/protein characterization data.      

RNA consistency

 Mainly not consistent with RNA expression data.      

WESTERN BLOT
 
 

Antibody HPA024268

  

Antibody HPA029255

  

Antibody HPA029256

  

Standard validation

 Uncertain

Analysis performed using a standard panel of samples.
No bands detected.		   Uncertain

Analysis performed using a standard panel of samples.
Weak band of predicted size but with additional bands of higher intensity also present.		   Uncertain

Analysis performed using a standard panel of samples.
Weak band of predicted size but with additional bands of higher intensity also present.		  

     

Figure description

 Lane 1: Marker [kDa] 230, 130, 95, 72, 56, 36, 28, 17, 11
Lane 2: RT4
Lane 3: U-251 MG
Lane 4: Human Plasma
Lane 5: Liver
Lane 6: Tonsil		      

Target mass (kDa)

 169.3, 135.7, 133.7, 129.6   169.3, 135.7, 133.7, 129.6   169.3, 135.7, 133.7, 129.6  

Antibody dilution

 1:250   1:250   1:250  

PROTEIN ARRAY
 
 

Antibody HPA024268

  

Antibody HPA029255

  

Antibody HPA029256

  

Standard validation

 Supported

Pass with single peak corresponding to interaction only with its own antigen.		   Supported

Pass with single peak corresponding to interaction only with its own antigen.		   Supported

Pass with single peak corresponding to interaction only with its own antigen.		  

     

Figure description

 Antibody specificity analysis with protein arrays. Predicted and matching interactions are shown in green.   Antibody specificity analysis with protein arrays. Predicted and matching interactions are shown in green.   Antibody specificity analysis with protein arrays. Predicted and matching interactions are shown in green.  

Antibody dilution

 1:500   1:30000   1:3000  

ANTIGEN INFORMATION
 
 

Antibody HPA024268

  

Antibody HPA029255

  

Antibody HPA029256

  

Antigen

 Recombinant protein fragment   Recombinant protein fragment   Recombinant protein fragment  

Length (aa)

 132   73   76  

Antigen sequence

 
KHVQLTHDKVEAQSCQLCGTKVSTRASMSRHMRRKHPEVLAVRIDDLDHL
PETTTIDASSIGIVQPELTLEQEDLAEGKHGKAAKRSHKRKQKPEEEAGA
PVPEDATFSEYSEKETEFTGSVGDETNSAVQS
  
ATQFTNLQPVAVGHLTTPERQLQLDNSILTVTFDTVSGSAMLHNRQNDVQ
IHPQPEASNPQSVAHFINLTTLV
  
DKPMLKQAGSGVHAAGTPENSAPVESEPSQWACKVCSATFLELQLLNEHL
LGHLEQAKSLPPGSQSEAAAPEKEQD
 

Matching transcripts

 PRDM15-010 - ENSP00000408592 [100%]
PRDM15-018 - ENSP00000381556 [100%]
PRDM15-201 - ENSP00000269844 [100%]
PRDM15-202 - ENSP00000390245 [100%]
  PRDM15-010 - ENSP00000408592 [100%]
PRDM15-018 - ENSP00000381556 [100%]
PRDM15-201 - ENSP00000269844 [100%]
PRDM15-202 - ENSP00000390245 [100%]
  PRDM15-010 - ENSP00000408592 [100%]
PRDM15-018 - ENSP00000381556 [100%]
PRDM15-201 - ENSP00000269844 [100%]
PRDM15-202 - ENSP00000390245 [100%]
 

Other gene match

      
ANTIGEN VIEW
PRDM15-010
PRDM15-018
PRDM15-201
PRDM15-202
 

Contact

The Project

The Human Protein Atlas

The Human Protein Atlas project is funded
by the Knut & Alice Wallenberg foundation.