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Pearson correlation >0.6 for protein expression in cell lines using independent antibodies.
Validated
Pearson correlation >0.6 for protein expression in cell lines using independent antibodies.
Figure description
Distribution of protein expression (antibody staining). Pearson correlation with HPA029401 across 45 cell lines.
Distribution of protein expression (antibody staining). Pearson correlation with HPA019142 across 45 cell lines.
Standard validation
Supported
Supported
Figure description
Immunohistochemical staining of human testis shows distinct positivity in collagen.
Immunohistochemical staining of human testis shows distinct positivity in extracellular matrix.
Expression
RNA: detected in 36 tissues Protein: detected in 12 cell types
RNA: detected in 36 tissues Protein: detected in 6 cell types
Retrieval
HIER pH6
HIER pH6
Antibody dilution
1:1000
1:150
Literature conformity
Consistent with extensive gene/protein characterization data.
Consistent with extensive gene/protein characterization data.
RNA consistency
Consistent with RNA expression data.
Consistent with RNA expression data.
WESTERN BLOT
Antibody HPA019142
Antibody HPA029401
Standard validation
Uncertain
Analysis performed using a standard panel of samples. Single band larger than predicted size in kDa (+20%) but partly supported by experimental and/or bioinformatic data.
Uncertain
Analysis performed using a standard panel of samples. Weak band of predicted size but with additional bands of higher intensity also present.
Figure description
Lane 1: Marker [kDa] 230, 130, 95, 72, 56, 36, 28, 17, 11 Lane 2: RT4 Lane 3: U-251 MG Lane 4: Human Plasma Lane 5: Liver Lane 6: Tonsil
Target mass (kDa)
108.5, 108.3
108.5, 108.3
Antibody dilution
1:250
1:250
PROTEIN ARRAY
Antibody HPA019142
Antibody HPA029401
Standard validation
Supported
Pass with single peak corresponding to interaction only with its own antigen.
Supported
Pass with single peak corresponding to interaction only with its own antigen.
Figure description
Antibody specificity analysis with protein arrays. Predicted and matching interactions are shown in green.
Antibody specificity analysis with protein arrays. Predicted and matching interactions are shown in green.
