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SLC6A3

ANTIBODY INFORMATION
 
 

Antibody HPA013602

  

Antibody CAB016249

  

Antibody CAB078172

  

Provider

 Atlas Antibodies
Sigma-Aldrich
   Santa Cruz Biotechnology
   Atlas Antibodies
Sigma-Aldrich
  

Product name

 HPA013602   sc-32259   AMAb91125  

Host species

 Rabbit   Rat   Mouse  

Clonality

 pAb   mAb   mAb  

Purity

 Affinity purified using the PrEST-antigen as affinity ligand   Protein A/G   Protein A/G  

Other gene match

      

Released in version

 4   4   16  

References

      

VALIDATION SUMMARY
 
 

Antibody HPA013602

  

Antibody CAB016249

  

Antibody CAB078172

  
Immunocytochemistry

Uncertain      
Immunohistochemistry

Uncertain   Uncertain    
Western blot

Uncertain   Uncertain    
Protein array

Supported      

IMMUNOCYTOCHEMISTRY
 
 

Antibody HPA013602

  

Antibody CAB016249

  

Antibody CAB078172

  

Standard validation

 Uncertain      

     

Figure description

 Immunofluorescent staining of human cell line U-2 OS shows localization to vesicles.      

Antibody dilution

 1:38      

Literature conformity

 The subcellular location is not consistent with literature.      

IMMUNOHISTOCHEMISTRY
 
 

Antibody HPA013602

  

Antibody CAB016249

  

Antibody CAB078172

  

Standard validation

 Uncertain   Uncertain    

     

Figure description

 Immunohistochemical staining of human liver shows strong cytoplasmic positivity in hepatocytes.   Immunohistochemical staining of human skeletal muscle shows strong cytoplasmic positivity in myocytes.    

Expression

 RNA: detected in 0 tissues
Protein: detected in 66 cell types		   RNA: detected in 0 tissues
Protein: detected in 56 cell types		    

Retrieval

 HIER pH6   HIER pH6    

Antibody dilution

 1:200   1:50    

Literature conformity

 Partly consistent with extensive gene/protein characterization data.   Partly consistent with extensive gene/protein characterization data.    

RNA consistency

 No internal RNA expression data available for correlation.   No internal RNA expression data available for correlation.    
 

Standard validation

     Supported  

     

Figure description

     Staining observed in neuronal axons/dendrites and synapses in selected brain regions of forebrain and midbrain.  

WESTERN BLOT
 
 

Antibody HPA013602

  

Antibody CAB016249

  

Antibody CAB078172

  

Standard validation

 Uncertain

Analysis performed using a standard panel of samples.
Current setup is not applicable due to low RNA count.		   Uncertain

Analysis performed using a standard panel of samples.
Current setup is not applicable due to low RNA count.		    

     

Figure description

      

Target mass (kDa)

 68.5   68.5    

Antibody dilution

 1:250   1:500    

PROTEIN ARRAY
 
 

Antibody HPA013602

  

Antibody CAB016249

  

Antibody CAB078172

  

Standard validation

 Supported

Pass with single peak corresponding to interaction only with its own antigen.		      

     

Figure description

 Antibody specificity analysis with protein arrays. Predicted and matching interactions are shown in green.      

Antibody dilution

 1:3000      

ANTIGEN INFORMATION
 
 

Antibody HPA013602

  

Antibody CAB016249

  

Antibody CAB078172

  

Antigen

 Recombinant protein fragment   Not known   Recombinant protein  

Length (aa)

 71      

Antigen sequence

 
LHYLFSSFTTELPWIHCNNSWNSPNCSDAHPGDSSGDSSGLNDTFGTTPA
AEYFERGVLHLHQSHGIDDLG
     

Matching transcripts

 SLC6A3-001 - ENSP00000270349 [100%]
     

Other gene match

      
ANTIGEN VIEW
SLC6A3-001
 

Contact

The Project

The Human Protein Atlas

The Human Protein Atlas project is funded
by the Knut & Alice Wallenberg foundation.