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SERBP1

ANTIBODY INFORMATION
 
 

Antibody HPA020559

  

Antibody CAB026297

  

Provider

 Atlas Antibodies
Sigma-Aldrich
   Abnova
  

Product name

 HPA020559   H00026135-M01  

Host species

 Rabbit   Mouse  

Clonality

 pAb   mAb  

Purity

 Affinity purified using the PrEST-antigen as affinity ligand   Protein A/G  

Other gene match

    

Released in version

 5   6  

References

 2    

VALIDATION SUMMARY
 
 

Antibody HPA020559

  

Antibody CAB026297

  
Immunocytochemistry


Validated
   Supported  
Immunohistochemistry

Supported   Supported  
Western blot

Supported   Supported  
Protein array

Supported    

IMMUNOCYTOCHEMISTRY
 
 

Antibody HPA020559

  

Antibody CAB026297

  

Formal validation: Genetic
Validated

Significant downregulation > 25% by one siRNA.		    

 

Relative fluorescence intensity (RFI)

    

Figure description

 Cytoplasmic region of segmented cells in 10x-images    

Antibody dilution

 1:32    
 

Standard validation

 Supported   Supported  

   

Figure description

 Immunofluorescent staining of human cell line U-2 OS shows localization to cytosol.   Immunofluorescent staining of human cell line U-251 MG shows localization to cytosol.  

Antibody dilution

 1:20   1:200  

Literature conformity

 The subcellular location is supported by literature.   The subcellular location is supported by literature.  

IMMUNOHISTOCHEMISTRY
 
 

Antibody HPA020559

  

Antibody CAB026297

  

Standard validation

 Supported   Supported  

   

Figure description

 Immunohistochemical staining of human cervix, uterine shows strong cytoplasmic positivity in squamous epithelial cells.   Immunohistochemical staining of human cervix, uterine shows strong cytoplasmic positivity in squamous epithelial cells.  

Expression

 RNA: detected in 37 tissues
Protein: detected in 80 cell types		   RNA: detected in 37 tissues
Protein: detected in 75 cell types		  

Retrieval

 HIER pH6   HIER pH6  

Antibody dilution

 1:150   1:800  

Literature conformity

 Partly consistent with extensive gene/protein characterization data.   Partly consistent with extensive gene/protein characterization data.  

RNA consistency

 Mainly consistent with RNA expression data.   Mainly consistent with RNA expression data.  

WESTERN BLOT
 
 

Antibody HPA020559

  

Antibody CAB026297

  

Standard validation

 Supported

Analysis performed using a standard panel of samples.
Single band corresponding to the predicted size in kDa (+/-20%).		   Supported

Analysis performed using a standard panel of samples.
Single band corresponding to the predicted size in kDa (+/-20%).		  

   

Figure description

 Lane 1: Marker [kDa] 230, 130, 95, 72, 56, 36, 28, 17, 11
Lane 2: RT4
Lane 3: U-251 MG
Lane 4: Human Plasma
Lane 5: Liver
Lane 6: Tonsil		   Lane 1: Marker [kDa] 250, 130, 95, 72, 55, 36, 28, 17, 11
Lane 2: RT4
Lane 3: U-251 MG
Lane 4: Human Plasma
Lane 5: Liver
Lane 6: Tonsil		  

Target mass (kDa)

 45, 44.3, 43.1, 42.4   45, 44.3, 43.1, 42.4  

Antibody dilution

 1:250   1:500  

PROTEIN ARRAY
 
 

Antibody HPA020559

  

Antibody CAB026297

  

Standard validation

 Supported

Pass with single peak corresponding to interaction only with its own antigen.		    

   

Figure description

 Antibody specificity analysis with protein arrays. Predicted and matching interactions are shown in green.    

Antibody dilution

 1:500    

ANTIGEN INFORMATION
 
 

Antibody HPA020559

  

Antibody CAB026297

  

Antigen

 Recombinant protein fragment   Recombinant protein  

Length (aa)

 137    

Antigen sequence

 
GHLQEGFGCVVTNRFDQLFDDESDPFEVLKAAENKKKEAGGGGVGGPGAK
SAAQAAAQTNSNAAGKQLRKESQKDRKNPLPPSVGVVDKKEETQPPVALK
KEGIRRVGRRPDQQLQGEGKIIDRRPERRPPRERRFE
   

Matching transcripts

 SERBP1-001 - ENSP00000360033 [100%]
SERBP1-002 - ENSP00000360034 [100%]
SERBP1-005 - ENSP00000354591 [100%]
SERBP1-006 - ENSP00000360029 [100%]
   

Other gene match

    
ANTIGEN VIEW
SERBP1-001
SERBP1-002
SERBP1-005
SERBP1-006

RELEVANT PUBLICATIONS
 
 

Antibody HPA020559

  

Antibody CAB026297

  

1.

 Matrix remodeling stimulates stromal autophagy, "fueling" cancer cell mitochondrial metabolism and metastasis
Castello-Cros R et al
Cell Cycle 2011;10(12):2021-34
    

2.

 Immunofluorescence and fluorescent-protein tagging show high correlation for protein localization in mammalian cells
Stadler C et al
Nat Methods 2013;10(4):315-23
    
 

Contact

The Project

The Human Protein Atlas

The Human Protein Atlas project is funded
by the Knut & Alice Wallenberg foundation.