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RPS8

ANTIBODY INFORMATION
 
 

Antibody HPA029266

  

Provider

 Atlas Antibodies
Sigma-Aldrich
  

Product name

 HPA029266  

Host species

 Rabbit  

Clonality

 pAb  

Purity

 Affinity purified using the PrEST-antigen as affinity ligand  

Other gene match

  

Released in version

 6  

References

  

VALIDATION SUMMARY
 
 

Antibody HPA029266

  
Immunocytochemistry


Validated
  
Western blot

Uncertain  
Protein array

Supported  

IMMUNOCYTOCHEMISTRY
 
 

Antibody HPA029266

  

Formal validation: Genetic
Validated

Significant downregulation > 25% by both siRNA:s.		  

 

Relative fluorescence intensity (RFI)

  

Figure description

 Cytoplasmic region of segmented cells in 10x-images  

Antibody dilution

 1:24  
 

Standard validation

 Supported  

 

Figure description

 Immunofluorescent staining of human cell line A-431 shows localization to cytosol & endoplasmic reticulum.  

Antibody dilution

 1:20  

Literature conformity

 The subcellular location is supported by literature.  

WESTERN BLOT
 
 

Antibody HPA029266

  

Standard validation

 Uncertain

Analysis performed using a standard panel of samples.
No bands detected.		  

 

Figure description

 Lane 1: Marker [kDa] 230, 130, 95, 72, 56, 36, 28, 17, 11
Lane 2: RT4
Lane 3: U-251 MG
Lane 4: Human Plasma
Lane 5: Liver
Lane 6: Tonsil		  

Target mass (kDa)

 24.2, 21.9  

Antibody dilution

 1:250  

PROTEIN ARRAY
 
 

Antibody HPA029266

  

Standard validation

 Supported

Pass with single peak corresponding to interaction only with its own antigen.		  

 

Figure description

 Antibody specificity analysis with protein arrays. Predicted and matching interactions are shown in green.  

Antibody dilution

 1:3000  

ANTIGEN INFORMATION
 
 

Antibody HPA029266

  

Antigen

 Recombinant protein fragment  

Length (aa)

 67  

Antigen sequence

 
AKLTPEEEEILNKKRSKKIQKKYDERKKNAKISSLLEEQFQQGKLLACIA
SRPGQCGRADGYVLEGK
 

Matching transcripts

 RPS8-001 - ENSP00000379888 [100%]
RPS8-003 - ENSP00000361283 [100%]
 

Other gene match

  
ANTIGEN VIEW
RPS8-001
RPS8-003
 

Contact

The Project

The Human Protein Atlas

The Human Protein Atlas project is funded
by the Knut & Alice Wallenberg foundation.