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STRIP1

ANTIBODY INFORMATION
 
 

Antibody HPA060302

  

Antibody HPA060498

  

Provider

 Atlas Antibodies
Sigma-Aldrich
   Atlas Antibodies
Sigma-Aldrich
  

Product name

 HPA060302   HPA060498  

Host species

 Rabbit   Rabbit  

Clonality

 pAb   pAb  

Purity

 Affinity purified using the PrEST-antigen as affinity ligand   Affinity purified using the PrEST-antigen as affinity ligand  

Other gene match

    

Released in version

 12   14  

References

    

VALIDATION SUMMARY
 
 

Antibody HPA060302

  

Antibody HPA060498

  
Immunocytochemistry

  Supported  
Immunohistochemistry

Approved    
Western blot

Uncertain   Supported  
Protein array

Approved   Supported  

IMMUNOCYTOCHEMISTRY
 
 

Antibody HPA060302

  

Antibody HPA060498

  

Standard validation

   Supported  

   

Figure description

   Immunofluorescent staining of human cell line RH-30 shows localization to cytosol.  

Antibody dilution

   1:69  

Literature conformity

   The subcellular location is supported by literature.  

IMMUNOHISTOCHEMISTRY
 
 

Antibody HPA060302

  

Antibody HPA060498

  

Standard validation

 Approved    

   

Figure description

 Immunohistochemical staining of human colon shows moderate cytoplasmic positivity in glandular cells.    

Expression

 RNA: detected in 37 tissues
Protein: detected in 50 cell types		    

Retrieval

 HIER pH6    

Antibody dilution

 1:20    

Literature conformity

 No avaliable gene/protein characterization data.    

RNA consistency

 Mainly not consistent with RNA expression data.    

WESTERN BLOT
 
 

Antibody HPA060302

  

Antibody HPA060498

  

Standard validation

 Uncertain

Analysis performed using a standard panel of samples.
No bands detected.		   Supported

Analysis performed using a standard panel of samples.
Single band corresponding to the predicted size in kDa (+/-20%).		  

   

Figure description

 Lane 1: Marker [kDa] 250, 130, 95, 72, 55, 36, 28, 17, 10
Lane 2: RT4
Lane 3: U-251 MG
Lane 4: Human Plasma
Lane 5: Liver
Lane 6: Tonsil		   Lane 1: Marker [kDa] 250, 130, 95, 72, 55, 36, 28, 17, 10
Lane 2: RT4
Lane 3: U-251 MG
Lane 4: Human Plasma
Lane 5: Liver
Lane 6: Tonsil		  

Target mass (kDa)

 95.6, 85.4   95.6, 85.4  

Antibody dilution

 1:120   1:230  

PROTEIN ARRAY
 
 

Antibody HPA060302

  

Antibody HPA060498

  

Standard validation

 Approved

Pass with quality comment low specificity (binding to 1-2 PrESTs >15% and <40%).		   Supported

Pass with single peak corresponding to interaction only with its own antigen.		  

   

Figure description

 Antibody specificity analysis with protein arrays. Predicted and matching interactions are shown in green.   Antibody specificity analysis with protein arrays. Predicted and matching interactions are shown in green.  

Antibody dilution

 1:1300   1:2750  

ANTIGEN INFORMATION
 
 

Antibody HPA060302

  

Antibody HPA060498

  

Antigen

 Recombinant protein fragment   Recombinant protein fragment  

Length (aa)

 53   60  

Antigen sequence

 
VLLNIMYLIVETVHQECEGDKAEWRTMRQTFRAELGSPLYNNEPFAIMLF
GMV
  
DSIKVIRNMRAASPPASASDLIEQQQKRGRREHKALIKQDNLDAFNERDP
YKADDSREEE
 

Matching transcripts

 STRIP1-001 - ENSP00000358810 [100%]
STRIP1-002 - ENSP00000358811 [100%]
  STRIP1-001 - ENSP00000358810 [100%]
STRIP1-002 - ENSP00000358811 [100%]
 

Other gene match

    
ANTIGEN VIEW
STRIP1-001
STRIP1-002
 

Contact

The Project

The Human Protein Atlas

The Human Protein Atlas project is funded
by the Knut & Alice Wallenberg foundation.