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Immunohistochemical staining of human ovary shows positivity in plasma.
Immunohistochemical staining of human skin shows positivity in plasma.
Expression
RNA: detected in 3 tissues Protein: detected in 30 cell types
RNA: detected in 3 tissues Protein: detected in 15 cell types
Retrieval
HIER pH6
HIER pH6
Antibody dilution
1:150
1:75
Literature conformity
Partly consistent with extensive gene/protein characterization data.
Partly consistent with extensive gene/protein characterization data.
RNA consistency
No internal RNA expression data available for correlation.
No internal RNA expression data available for correlation.
WESTERN BLOT
Antibody HPA003827
Antibody HPA052139
Standard validation
Supported
Analysis performed using a standard panel of samples. Band of predicted size in kDa (+/-20%) with additional bands present.
Uncertain
Analysis performed using a standard panel of samples. Single band larger than predicted size in kDa (+20%) but partly supported by experimental and/or bioinformatic data.
Figure description
Lane 1: Marker [kDa] 230, 110, 82, 49.3, 32.2, 25.5, 17.6 Lane 2: RT4 Lane 3: U-251 MG Lane 4: Human Plasma Lane 5: Liver Lane 6: Tonsil
Lane 1: Marker [kDa] 250, 130, 95, 72, 55, 36, 28, 17, 10 Lane 2: RT4 Lane 3: U-251 MG Lane 4: Human Plasma Lane 5: Liver Lane 6: Tonsil
Target mass (kDa)
75.5
75.5
Antibody dilution
1:250
1:460
PROTEIN ARRAY
Antibody HPA003827
Antibody HPA052139
Standard validation
Supported
Pass with single peak corresponding to interaction only with its own antigen.
Supported
Pass with single peak corresponding to interaction only with its own antigen.
Figure description
Antibody specificity analysis with protein arrays. Predicted and matching interactions are shown in green.
Antibody specificity analysis with protein arrays. Predicted and matching interactions are shown in green.
