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Pearson correlation >0.6 for protein expression in cell lines using independent antibodies.
Validated
Pearson correlation >0.6 for protein expression in cell lines using independent antibodies.
Validated
Pearson correlation >0.6 for protein expression in cell lines using independent antibodies.
Figure description
Distribution of protein expression (antibody staining). Pearson correlation with HPA027657 across 46 cell lines. Pearson correlation with HPA027587 across 46 cell lines.
Distribution of protein expression (antibody staining). Pearson correlation with HPA027586 across 46 cell lines.
Distribution of protein expression (antibody staining). Pearson correlation with HPA027586 across 46 cell lines.
Formal validation: Independent
Validated
Spearman correlation >0.6 for protein expression in tissues using independent antibodies.
Validated
Spearman correlation >0.6 for protein expression in tissues using independent antibodies.
Validated
Spearman correlation >0.6 for protein expression in tissues using independent antibodies.
Figure description
Distribution of protein expression (antibody staining). Spearman correlation with HPA027657 across 74 cell types. Spearman correlation with HPA027587 across 74 cell types.
Distribution of protein expression (antibody staining). Spearman correlation with HPA027586 across 74 cell types.
Distribution of protein expression (antibody staining). Spearman correlation with HPA027586 across 74 cell types.
Standard validation
Supported
Supported
Supported
Supported
Figure description
Immunohistochemical staining of human gall bladder shows distinct cytoplasmic and membranous positivity in glandular cells.
Immunohistochemical staining of human colon shows strong cytoplasmic and membranous positivity in glandular cells.
Immunohistochemical staining of human colon shows strong cytoplasmic and membranous positivity in glandular cells.
Immunohistochemical staining of human gall bladder shows cytoplasmic and membranous positivity in glandular cells.
Expression
RNA: detected in 31 tissues Protein: detected in 31 cell types
RNA: detected in 31 tissues Protein: detected in 41 cell types
RNA: detected in 31 tissues Protein: detected in 51 cell types
RNA: detected in 31 tissues Protein: detected in 72 cell types
Retrieval
HIER pH6
HIER pH6
HIER pH6
HIER pH6
Antibody dilution
1:1500
1:225
1:60
1:175
Literature conformity
Consistent with extensive gene/protein characterization data.
Partly consistent with extensive gene/protein characterization data.
Consistent with extensive gene/protein characterization data.
Partly consistent with extensive gene/protein characterization data.
RNA consistency
Mainly consistent with RNA expression data.
Mainly consistent with RNA expression data.
Mainly consistent with RNA expression data.
Mainly consistent with RNA expression data.
WESTERN BLOT
Antibody HPA027586
Antibody HPA027587
Antibody HPA027657
Antibody CAB017193
Standard validation
Supported
Analysis performed using a standard panel of samples. Band of predicted size in kDa (+/-20%) with additional bands present.
Uncertain
Analysis performed using a standard panel of samples. Only bands not corresponding to the predicted size.
Supported
Analysis performed using a standard panel of samples. Band of predicted size in kDa (+/-20%) with additional bands present.
Supported
Analysis performed using a standard panel of samples. Band of predicted size in kDa (+/-20%) with additional bands present.
Figure description
Lane 1: Marker [kDa] 230, 130, 95, 72, 56, 36, 28, 17, 11 Lane 2: RT4 Lane 3: U-251 MG Lane 4: Human Plasma Lane 5: Liver Lane 6: Tonsil
Lane 1: Marker [kDa] 230, 130, 95, 72, 56, 36, 28, 17, 11 Lane 2: RT4 Lane 3: U-251 MG Lane 4: Human Plasma Lane 5: Liver Lane 6: Tonsil
Lane 1: Marker [kDa] 250, 130, 95, 72, 55, 36, 28, 17, 11 Lane 2: RT4 Lane 3: U-251 MG Lane 4: Human Plasma Lane 5: Liver Lane 6: Tonsil
Target mass (kDa)
137.1, 14.8
137.1, 31.5, 17.6
137.1
137.1, 31.5, 17.6, 14.8
Antibody dilution
1:250
1:250
1:250
1:500
PROTEIN ARRAY
Antibody HPA027586
Antibody HPA027587
Antibody HPA027657
Antibody CAB017193
Standard validation
Supported
Pass with single peak corresponding to interaction only with its own antigen.
Supported
Pass with single peak corresponding to interaction only with its own antigen.
Supported
Pass with single peak corresponding to interaction only with its own antigen.
Figure description
Antibody specificity analysis with protein arrays. Predicted and matching interactions are shown in green.
Antibody specificity analysis with protein arrays. Predicted and matching interactions are shown in green.
Antibody specificity analysis with protein arrays. Predicted and matching interactions are shown in green.
