SETDB1 - Antibodies - The Human Protein Atlas

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SETDB1

TISSUE

CELL

CANCER

ANTIBODY INFORMATION

Antibody HPA018142

Antibody HPA058484

Provider

Atlas Antibodies
Sigma-Aldrich

Product name

HPA018142

HPA058484

Host species

Rabbit

Clonality

pAb

Purity

Affinity purified using the PrEST-antigen as affinity ligand

Other gene match

Released in version

References

VALIDATION SUMMARY

Antibody HPA018142

Antibody HPA058484

Immunocytochemistry

Immunohistochemistry

Western blot

Protein array

IMMUNOCYTOCHEMISTRY

Antibody HPA018142

Antibody HPA058484

Standard validation

Supported

Figure description

Immunofluorescent staining of human cell line A-431 shows localization to nucleoplasm, plasma membrane & cytosol.

Immunofluorescent staining of human cell line U-251 MG shows localization to nucleoplasm.

Antibody dilution

1:200

1:128

Literature conformity

The subcellular location is supported by literature.

IMMUNOHISTOCHEMISTRY

Antibody HPA018142

Antibody HPA058484

Standard validation

Uncertain

Figure description

Immunohistochemical staining of human colon shows strong nuclear, cytoplasmic and membranous positivity in glandular cells.

Expression

RNA: detected in 37 tissues
Protein: detected in 52 cell types

Retrieval

HIER pH6

Antibody dilution

1:550

Literature conformity

Partly consistent with extensive gene/protein characterization data.

RNA consistency

Mainly not consistent with RNA expression data.

WESTERN BLOT

Antibody HPA018142

Antibody HPA058484

Standard validation

Supported

Analysis performed using a standard panel of samples.
Band of predicted size in kDa (+/-20%) with additional bands present.

Uncertain

Analysis performed using a standard panel of samples.
Only bands not corresponding to the predicted size.

Figure description

Lane 1: Marker [kDa] 230, 130, 95, 72, 56, 36, 28, 17, 11
Lane 2: RT4
Lane 3: U-251 MG
Lane 4: Human Plasma
Lane 5: Liver
Lane 6: Tonsil

Target mass (kDa)

143.2, 143, 139.4, 71

143.2, 143, 139.4

Antibody dilution

1:250

1:430

PROTEIN ARRAY

Antibody HPA018142

Antibody HPA058484

Standard validation

Supported

Pass with single peak corresponding to interaction only with its own antigen.

Figure description

Antibody specificity analysis with protein arrays. Predicted and matching interactions are shown in green.

Antibody dilution

1:3000

1:5100

ANTIGEN INFORMATION

Antibody HPA018142

Antibody HPA058484

Antigen

Recombinant protein fragment

Length (aa)

148

Antigen sequence

KLRFLIFFDDGYASYVTQSELYPICRPLKKTWEDIEDISCRDFIEEYVTA
YPNRPMVLLKSGQLIKTEWEGTWWKSRVEEVDGSLVRILFLDDKRCEWIY
RGSTRLEPMFSMKTSSASALEKKQGQLRTRPNMGAVRSKGPVVQYTQD

SGTSRKPTAGQTSATAVDSDDIQTISSGSEGDDFEDKKNMTGPMKRQVAV
KSTRGFALKSTHGIAIKSTNMASVDKGESAPVRKNTRQFYDGEESCY

Matching transcripts

SETDB1-001 - ENSP00000271640 [100%]
SETDB1-005 - ENSP00000432348 [100%]
SETDB1-013 - ENSP00000436148 [100%]
SETDB1-014 - ENSP00000357965 [100%]

SETDB1-001 - ENSP00000271640 [100%]
SETDB1-005 - ENSP00000432348 [100%]
SETDB1-014 - ENSP00000357965 [100%]

Other gene match

ANTIGEN VIEW

SETDB1-001	SETDB1-005	SETDB1-013	SETDB1-014

RELEVANT PUBLICATIONS

Antibody HPA018142

Antibody HPA058484

The histone methyltransferase SETDB1 is recurrently amplified in melanoma and accelerates its onset
Ceol CJ et al
Nature 2011;471(7339):513-7

Gene amplification of the histone methyltransferase SETDB1 contributes to human lung tumorigenesis
Rodriguez-Paredes M et al
Oncogene 2014;33(21):2807-13

The histone methyltransferase SETDB1 is recurrently amplified in melanoma and accelerates its onset
Ceol CJ et al
Nature 2011;471(7339):513-7

Contact

contact@proteinatlas.org

The Project

The Human Protein Atlas

The Human Protein Atlas project is funded
by the Knut & Alice Wallenberg foundation.