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Immunohistochemical staining of human lymph node shows strong nuclear positivity in reaction center cells as well as in lymphoid cells outside reaction center.
Expression
RNA: detected in 37 tissues Protein: detected in 79 cell types
Retrieval
HIER pH6
Antibody dilution
1:75
Literature conformity
Consistent with extensive gene/protein characterization data.
RNA consistency
Mainly consistent with RNA expression data.
WESTERN BLOT
Antibody HPA018519
Standard validation
Uncertain
Analysis performed using a standard panel of samples. Weak band of predicted size but with additional bands of higher intensity also present.
Figure description
Target mass (kDa)
65.3, 63.1, 25.8
Antibody dilution
1:250
PROTEIN ARRAY
Antibody HPA018519
Standard validation
Supported
Pass with single peak corresponding to interaction only with its own antigen.
Figure description
Antibody specificity analysis with protein arrays. Predicted and matching interactions are shown in green.
