PKLR - Antibodies - The Human Protein Atlas

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PKLR

TISSUE

CELL

CANCER

ANTIBODY INFORMATION

Antibody HPA006653

Antibody CAB034376

Antibody CAB034378

Provider

Atlas Antibodies
Sigma-Aldrich

Santa Cruz Biotechnology

Product name

HPA006653

sc-133222

sc-133224

Host species

Rabbit

Mouse

Clonality

pAb

mAb

Purity

Affinity purified using the PrEST-antigen as affinity ligand

Protein A/G

Other gene match

Released in version

References

VALIDATION SUMMARY

Antibody HPA006653

Antibody CAB034376

Antibody CAB034378

Immunocytochemistry

Approved

Immunohistochemistry

Western blot

Supported

Protein array

Supported

IMMUNOCYTOCHEMISTRY

Antibody HPA006653

Antibody CAB034376

Antibody CAB034378

Standard validation

Approved

Figure description

Immunofluorescent staining of human cell line Hep G2 shows localization to cytosol.

Antibody dilution

1:5

Literature conformity

The subcellular location is partly supported by literature or no literature is available.

IMMUNOHISTOCHEMISTRY

Antibody HPA006653

Antibody CAB034376

Antibody CAB034378

Formal validation: Orthogonal

Validated

Pearson correlation >0.6 for protein and RNA expression in cell lines.

Validated

Pearson correlation >0.6 for protein and RNA expression in cell lines.

Figure description

Distribution of protein expression (antibody staining) and RNA expression (TPM) across 44 cell lines.

Formal validation: Orthogonal

Validated

Spearman correlation >0.6 for protein and RNA expression in tissues.

Validated

Spearman correlation >0.6 for protein and RNA expression in tissues.

Figure description

Distribution of protein expression (antibody staining) and RNA expression (TPM) across 37 tissues.

Standard validation

Supported

Figure description

Immunohistochemical staining of human liver shows strong cytoplasmic positivity in hepatocytes.

Expression

RNA: detected in 5 tissues
Protein: detected in 11 cell types

RNA: detected in 5 tissues
Protein: detected in 6 cell types

Retrieval

HIER pH6

Antibody dilution

1:4000

1:1000

Literature conformity

Consistent with extensive gene/protein characterization data.

RNA consistency

Consistent with RNA expression data.

WESTERN BLOT

Antibody HPA006653

Antibody CAB034376

Antibody CAB034378

Standard validation

Supported

Analysis performed using a standard panel of samples.
Single band corresponding to the predicted size in kDa (+/-20%).

Supported

Analysis performed using a standard panel of samples.
Band of predicted size in kDa (+/-20%) with additional bands present.

Figure description

Lane 1: Marker [kDa] 230, 130, 95, 72, 56, 36, 28, 17, 11
Lane 2: RT4
Lane 3: U-251 MG
Lane 4: Human Plasma
Lane 5: Liver
Lane 6: Tonsil

Lane 1: Marker [kDa] 250, 130, 100, 70, 55, 35, 25, 15, 10
Lane 2: RT4
Lane 3: U-251 MG
Lane 4: Human Plasma
Lane 5: Liver
Lane 6: Tonsil

Target mass (kDa)

61.8, 58.5

Antibody dilution

1:250

1:500

PROTEIN ARRAY

Antibody HPA006653

Antibody CAB034376

Antibody CAB034378

Standard validation

Supported

Pass with single peak corresponding to interaction only with its own antigen.

Figure description

Antibody specificity analysis with protein arrays. Predicted and matching interactions are shown in green.

Antibody dilution

1:500

ANTIGEN INFORMATION

Antibody HPA006653

Antibody CAB034376

Antibody CAB034378

Antigen

Recombinant protein fragment

Synthetic peptide

Length (aa)

115

Antigen sequence

GSPLSYRPVAIALDTKGPEIRTGILQGGPESEVELVKGSQVLVTVDPAFR
TRGNANTVWVDYPNIVRVVPVGGRIYIDDGLISLVVQKIGPEGLVTQVEN
GGVLGSRKGVNLPGA

Matching transcripts

PKLR-001 - ENSP00000339933 [100%]
PKLR-002 - ENSP00000376214 [100%]

Other gene match

ANTIGEN VIEW

PKLR-001	PKLR-002

Contact

contact@proteinatlas.org

The Project

The Human Protein Atlas

The Human Protein Atlas project is funded
by the Knut & Alice Wallenberg foundation.