We use cookies to enhance the usability of our website. If you continue, we'll assume that you are happy to receive all cookies. More information. Don't show this again.
Antibody staining overlaps with antibody HPA030188.
Validated
Antibody staining overlaps with antibody HPA027505.
Standard validation
Supported
Supported
Figure description
Immunofluorescent staining of human cell line HaCaT shows localization to vesicles.
Immunofluorescent staining of human cell line HaCaT shows localization to vesicles.
Antibody dilution
1:90
1:200
Literature conformity
The subcellular location is supported by literature.
The subcellular location is supported by literature.
IMMUNOHISTOCHEMISTRY
Antibody HPA027505
Antibody HPA030188
Antibody HPA030189
Antibody CAB002210
Standard validation
Supported
Supported
Supported
Figure description
Immunohistochemical staining of human skin shows strong positivity in epidermal cells.
Immunohistochemical staining of human vulva/anal skin shows strong cytoplasmic positivity in superficial layer of squamous epithelium.
Immunohistochemical staining of human skin shows strong cytoplasmic positivity in keratinized layer of epidermal cells.
Expression
RNA: detected in 5 tissues Protein: detected in 17 cell types
RNA: detected in 5 tissues Protein: detected in 7 cell types
RNA: detected in 5 tissues Protein: detected in 7 cell types
Retrieval
HIER pH6
HIER pH6
HIER pH6
Antibody dilution
1:3500
1:1500
1:75
Literature conformity
Consistent with extensive gene/protein characterization data.
Consistent with extensive gene/protein characterization data.
Consistent with extensive gene/protein characterization data.
RNA consistency
Mainly consistent with RNA expression data.
Mainly consistent with RNA expression data.
Mainly consistent with RNA expression data.
WESTERN BLOT
Antibody HPA027505
Antibody HPA030188
Antibody HPA030189
Antibody CAB002210
Standard validation
Uncertain
Analysis performed using a standard panel of samples. Single band differing more than +/-20% from predicted size in kDa and not supported by experimental and/or bioinformatic data.
Uncertain
Analysis performed using a standard panel of samples. Target too small/large to be analyzed with the present setup.
Uncertain
Analysis performed using a standard panel of samples. Target too small/large to be analyzed with the present setup.
Uncertain
Analysis performed using a standard panel of samples. No bands detected.
Figure description
Lane 1: Marker [kDa] 230, 130, 95, 72, 56, 36, 28, 17, 11 Lane 2: RT4 Lane 3: U-251 MG Lane 4: Human Plasma Lane 5: Liver Lane 6: Tonsil
Lane 1: Marker [kDa] 250, 130, 95, 72, 55, 36, 28, 17, 11 Lane 2: RT4 Lane 3: U-251 MG Lane 4: Human Plasma Lane 5: Liver Lane 6: Tonsil
Target mass (kDa)
435.2
435.2
435.2
435.2
Antibody dilution
1:250
1:250
1:250
1:500
PROTEIN ARRAY
Antibody HPA027505
Antibody HPA030188
Antibody HPA030189
Antibody CAB002210
Standard validation
Supported
Pass with single peak corresponding to interaction only with its own antigen.
Supported
Pass with single peak corresponding to interaction only with its own antigen.
Supported
Pass with single peak corresponding to interaction only with its own antigen.
Figure description
Antibody specificity analysis with protein arrays. Predicted and matching interactions are shown in green.
Antibody specificity analysis with protein arrays. Predicted and matching interactions are shown in green.
Antibody specificity analysis with protein arrays. Predicted and matching interactions are shown in green.
