EML4 - Antibodies - The Human Protein Atlas

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EML4

TISSUE

CELL

CANCER

ANTIBODY INFORMATION

Antibody HPA036687

Antibody HPA036688

Antibody HPA065337

Provider

Atlas Antibodies
Sigma-Aldrich

Product name

HPA036687

HPA036688

HPA065337

Host species

Rabbit

Clonality

pAb

Purity

Affinity purified using the PrEST-antigen as affinity ligand

Other gene match

Released in version

References

VALIDATION SUMMARY

Antibody HPA036687

Antibody HPA036688

Antibody HPA065337

Immunocytochemistry

Approved

Immunohistochemistry

Western blot

Protein array

IMMUNOCYTOCHEMISTRY

Antibody HPA036687

Antibody HPA036688

Antibody HPA065337

Standard validation

Approved

Figure description

Immunofluorescent staining of human cell line U-251 MG shows localization to cytosol, intermediate filaments & microtubules.

Antibody dilution

1:200

Literature conformity

The subcellular location is partly supported by literature or no literature is available.

IMMUNOHISTOCHEMISTRY

Antibody HPA036687

Antibody HPA036688

Antibody HPA065337

Formal validation: Orthogonal

Validated

Pearson correlation >0.6 for protein and RNA expression in cell lines.

Figure description

Distribution of protein expression (antibody staining) and RNA expression (TPM) across 44 cell lines.

Formal validation: Independent

Validated

Spearman correlation >0.6 for protein expression in tissues using independent antibodies.

Validated

Spearman correlation >0.6 for protein expression in tissues using independent antibodies.

Figure description

Distribution of protein expression (antibody staining).
Spearman correlation with HPA036688 across 75 cell types.

Distribution of protein expression (antibody staining).
Spearman correlation with HPA036687 across 75 cell types.

Standard validation

Supported

Figure description

Immunohistochemical staining of human small intestine shows strong cytoplasmic positivity in glandular cells.

Immunohistochemical staining of human colon shows strong cytoplasmic positivity in glandular cells.

Immunohistochemical staining of human duodenum shows strong cytoplasmic positivity in glandular cells.

Expression

RNA: detected in 36 tissues
Protein: detected in 63 cell types

RNA: detected in 36 tissues
Protein: detected in 71 cell types

RNA: detected in 36 tissues
Protein: detected in 44 cell types

Retrieval

HIER pH6

Antibody dilution

1:200

1:2000

1:3000

Literature conformity

Consistent with gene/protein characterization data.

RNA consistency

Mainly consistent with RNA expression data.

Mainly not consistent with RNA expression data.

Mainly consistent with RNA expression data.

WESTERN BLOT

Antibody HPA036687

Antibody HPA036688

Antibody HPA065337

Standard validation

Uncertain

Analysis performed using a standard panel of samples.
Weak band of predicted size but with additional bands of higher intensity also present.

Supported

Analysis performed using a standard panel of samples.
Band of predicted size in kDa (+/-20%) with additional bands present.

Uncertain

Analysis performed using a standard panel of samples.
Only bands not corresponding to the predicted size.

Figure description

Lane 1: Marker [kDa] 230, 130, 95, 72, 56, 36, 28, 17, 11
Lane 2: RT4
Lane 3: U-251 MG
Lane 4: Human Plasma
Lane 5: Liver
Lane 6: Tonsil

Target mass (kDa)

110.2, 108.9

110.2, 108.9, 102.4

Antibody dilution

1:250

1:510

PROTEIN ARRAY

Antibody HPA036687

Antibody HPA036688

Antibody HPA065337

Standard validation

Approved

Pass with quality comment low specificity (binding to 1-2 PrESTs >15% and <40%).

Supported

Pass with single peak corresponding to interaction only with its own antigen.

Figure description

Antibody specificity analysis with protein arrays. Predicted and matching interactions are shown in green.

Antibody dilution

1:3000

1:6150

ANTIGEN INFORMATION

Antibody HPA036687

Antibody HPA036688

Antibody HPA065337

Antigen

Recombinant protein fragment

Length (aa)

Antigen sequence

PQPSSQPLQIHRQTPESKNATPTKSIKRPSPAEKSHNSWENSDDSRNKLS
KIPSTPKLIPKVTKTADKHKDVIINQEGEY

MSIIQWKLVEKLSLPQNETVADTTLTKAPVSSTESVIQSNTPTPPPSQPL
NETAEEESRISSSPTLLENSLEQTVEPSEDHSEEES

GGVMLIWSKTTVEPTPGKGPKGVYQISKQIKAHDGSVFTLCQMRNGMLLT
GGGKDRKIILWDHDLNPEREIEVPDQYGTI

Matching transcripts

EML4-001 - ENSP00000320663 [100%]
EML4-003 - ENSP00000384939 [88%]

EML4-001 - ENSP00000320663 [100%]
EML4-002 - ENSP00000385059 [100%]
EML4-003 - ENSP00000384939 [100%]

Other gene match

ANTIGEN VIEW

EML4-001	EML4-002	EML4-003

RELEVANT PUBLICATIONS

Antibody HPA036687

Antibody HPA036688

Antibody HPA065337

Neural differentiation modulates the vertebrate brain specific splicing program
Madgwick A et al
PLoS One 2015;10(5):e0125998

Contact

contact@proteinatlas.org

The Project

The Human Protein Atlas

The Human Protein Atlas project is funded
by the Knut & Alice Wallenberg foundation.