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Immunofluorescent staining of human cell line U-2 OS shows localization to mitochondria.
Antibody dilution
1:38
Literature conformity
The subcellular location is partly supported by literature or no literature is available.
IMMUNOHISTOCHEMISTRY
Antibody HPA010639
Antibody HPA013434
Standard validation
Approved
Approved
Figure description
Immunohistochemical staining of human cerebral cortex shows strong cytoplasmic positivity in neuronal cells.
Immunohistochemical staining of human cerebral cortex shows moderate cytoplasmic positivity in neuronal cells.
Expression
RNA: detected in 32 tissues Protein: detected in 67 cell types
RNA: detected in 32 tissues Protein: detected in 72 cell types
Retrieval
HIER pH6
HIER pH6
Antibody dilution
1:75
1:50
Literature conformity
No avaliable gene/protein characterization data.
No avaliable gene/protein characterization data.
RNA consistency
Mainly not consistent with RNA expression data.
Mainly not consistent with RNA expression data.
WESTERN BLOT
Antibody HPA010639
Antibody HPA013434
Standard validation
Supported
Single band corresponding to the predicted size in kDa (+/-20%).
Uncertain
No bands detected.
Figure description
Lane 1: Marker [kDa] 250, 130, 95, 72, 55, 36, 28, 17, 10 Lane 2: Negative control (vector only transfected HEK293T lysate) Lane 3: Over-expression Lysate (Co-expressed with a C-terminal myc-DDK tag (~3.1 kDa) in mammalian HEK293T cells, LY406170)
Lane 1: Marker [kDa] 250, 130, 95, 72, 55, 36, 28, 17, 10 Lane 2: Negative control (vector only transfected HEK293T lysate) Lane 3: Over-expression Lysate (Co-expressed with a C-terminal myc-DDK tag (~3.1 kDa) in mammalian HEK293T cells, LY406170)
Target mass (kDa)
92.2
92.2
Antibody dilution
1:125
1:125
PROTEIN ARRAY
Antibody HPA010639
Antibody HPA013434
Standard validation
Supported
Pass with single peak corresponding to interaction only with its own antigen.
Supported
Pass with single peak corresponding to interaction only with its own antigen.
Figure description
Antibody specificity analysis with protein arrays. Predicted and matching interactions are shown in green.
Antibody specificity analysis with protein arrays. Predicted and matching interactions are shown in green.
