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Immunofluorescent staining of human cell line U-2 OS shows localization to nucleus & nucleoli fibrillar center.
Antibody dilution
1:8
Literature conformity
The subcellular location is partly supported by literature or no literature is available.
IMMUNOHISTOCHEMISTRY
Antibody HPA038225
Antibody HPA038614
Standard validation
Approved
Approved
Figure description
Immunohistochemical staining of human cerebral cortex shows moderate nuclear positivity in neuronal cells.
Immunohistochemical staining of human prostate shows moderate nuclear positivity in glandular cells.
Expression
RNA: detected in 37 tissues Protein: detected in 57 cell types
RNA: detected in 37 tissues Protein: detected in 54 cell types
Retrieval
HIER pH6
HIER pH6
Antibody dilution
1:10
1:50
Literature conformity
Consistent with gene/protein characterization data.
Consistent with gene/protein characterization data.
RNA consistency
Mainly consistent with RNA expression data.
Mainly consistent with RNA expression data.
WESTERN BLOT
Antibody HPA038225
Antibody HPA038614
Standard validation
Supported
Band of predicted size in kDa (+/-20%) with additional bands present.
Supported
Analysis performed using a standard panel of samples. Band of predicted size in kDa (+/-20%) with additional bands present.
Figure description
Lane 1: Marker [kDa] 250, 130, 95, 72, 55, 36, 28, 17, 10 Lane 2: Negative control (vector only transfected HEK293T lysate) Lane 3: Over-expression Lysate (Co-expressed with a C-terminal myc-DDK tag (~3.1 kDa) in mammalian HEK293T cells, LY415121)
Lane 1: Marker [kDa] 250, 130, 95, 72, 55, 36, 28, 17, 10 Lane 2: RT4 Lane 3: U-251 MG Lane 4: Human Plasma Lane 5: Liver Lane 6: Tonsil
