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VWA5B2

ANTIBODY INFORMATION
 
 

Antibody HPA036823

  

Antibody HPA036824

  

Antibody HPA061412

  

Provider

 Atlas Antibodies
Sigma-Aldrich
   Atlas Antibodies
Sigma-Aldrich
   Atlas Antibodies
Sigma-Aldrich
  

Product name

 HPA036823   HPA036824   HPA061412  

Host species

 Rabbit   Rabbit   Rabbit  

Clonality

 pAb   pAb   pAb  

Purity

 Affinity purified using the PrEST-antigen as affinity ligand   Affinity purified using the PrEST-antigen as affinity ligand   Affinity purified using the PrEST-antigen as affinity ligand  

Other gene match

      

Released in version

 7   13   13  

References

 1      

VALIDATION SUMMARY
 
 

Antibody HPA036823

  

Antibody HPA036824

  

Antibody HPA061412

  
Immunocytochemistry

Approved      
Immunohistochemistry

Approved   Approved   Approved  
Western blot

Uncertain   Uncertain   Uncertain  
Protein array

Supported   Approved   Supported  

IMMUNOCYTOCHEMISTRY
 
 

Antibody HPA036823

  

Antibody HPA036824

  

Antibody HPA061412

  

Standard validation

 Approved      

     

Figure description

 Immunofluorescent staining of human cell line U-2 OS shows localization to nucleus, nucleoli & the Golgi apparatus.      

Antibody dilution

 1:139      

Literature conformity

 The subcellular location is partly supported by literature or no literature is available.      

IMMUNOHISTOCHEMISTRY
 
 

Antibody HPA036823

  

Antibody HPA036824

  

Antibody HPA061412

  

Standard validation

 Approved   Approved   Approved  

     

Figure description

 Immunohistochemical staining of human colon shows strong cytoplasmic positivity, seen with a granular pattern, in glandular cells.   Immunohistochemical staining of human stomach, upper shows moderate cytoplasmic positivity in glandular cells.   Immunohistochemical staining of human pancreas shows strong cytoplasmic positivity in islets of Langerhans .  

Expression

 RNA: detected in 10 tissues
Protein: detected in 25 cell types		   RNA: detected in 10 tissues
Protein: detected in 61 cell types		   RNA: detected in 10 tissues
Protein: detected in 41 cell types		  

Retrieval

 HIER pH6   HIER pH6   HIER pH6  

Antibody dilution

 1:250   1:250   1:150  

Literature conformity

 No avaliable gene/protein characterization data.   No avaliable gene/protein characterization data.   No avaliable gene/protein characterization data.  

RNA consistency

 Mainly not consistent with RNA expression data.   Mainly consistent with RNA expression data.   Mainly consistent with RNA expression data.  

WESTERN BLOT
 
 

Antibody HPA036823

  

Antibody HPA036824

  

Antibody HPA061412

  

Standard validation

 Uncertain

Analysis performed using a standard panel of samples.
No bands detected.		   Uncertain

Analysis performed using a standard panel of samples.
Weak band of predicted size but with additional bands of higher intensity also present.		   Uncertain

Analysis performed using a standard panel of samples.
Only bands not corresponding to the predicted size.		  

     

Figure description

 Lane 1: Marker [kDa] 230, 130, 95, 72, 56, 36, 28, 17, 11
Lane 2: RT4
Lane 3: U-251 MG
Lane 4: Human Plasma
Lane 5: Liver
Lane 6: Tonsil		      

Target mass (kDa)

 131.7, 108.6   131.7, 108.6   131.7, 108.6  

Antibody dilution

 1:250   1:250   1:210  

PROTEIN ARRAY
 
 

Antibody HPA036823

  

Antibody HPA036824

  

Antibody HPA061412

  

Standard validation

 Supported

Pass with single peak corresponding to interaction only with its own antigen.		   Approved

Pass with quality comment low specificity (binding to 1-2 PrESTs >15% and <40%).		   Supported

Pass with single peak corresponding to interaction only with its own antigen.		  

     

Figure description

 Antibody specificity analysis with protein arrays. Predicted and matching interactions are shown in green.   Antibody specificity analysis with protein arrays. Predicted and matching interactions are shown in green.   Antibody specificity analysis with protein arrays. Predicted and matching interactions are shown in green.  

Antibody dilution

 1:3000   1:3000   1:2500  

ANTIGEN INFORMATION
 
 

Antibody HPA036823

  

Antibody HPA036824

  

Antibody HPA061412

  

Antigen

 Recombinant protein fragment   Recombinant protein fragment   Recombinant protein fragment  

Length (aa)

 82   94   79  

Antigen sequence

 
PRKPSLGAILDGPSPEPGQQLGQGLDDSGNLLSPAPMDWDMLMEPPFLFT
AVPPSGELAPPAVPPQAPRCHVVIRGLCGEQP
  
IVLAVKSLPPQTLINLAVFGTLVQPLFPESRPCSDDAVQLICESIETLQV
PSGPPDVLAALDWAVGQPQHRAYPRQLFLLTAASPMAATTHRTL
  
LPTVVYSKGLQRGSPAGAWDSDQNGNSKRALGDPATPTEGPRRPPPRPPC
RLSMGRRHKLCSPDPGQANNSEGSDHDYL
 

Matching transcripts

 VWA5B2-001 - ENSP00000398688 [100%]
VWA5B2-006 - ENSP00000273794 [100%]
  VWA5B2-001 - ENSP00000398688 [100%]
VWA5B2-006 - ENSP00000273794 [100%]
  VWA5B2-001 - ENSP00000398688 [100%]
VWA5B2-006 - ENSP00000273794 [100%]
 

Other gene match

      
ANTIGEN VIEW
VWA5B2-001
VWA5B2-006

RELEVANT PUBLICATIONS
 
 

Antibody HPA036823

  

Antibody HPA036824

  

Antibody HPA061412

  

1.

 Immunofluorescence and fluorescent-protein tagging show high correlation for protein localization in mammalian cells
Stadler C et al
Nat Methods 2013;10(4):315-23
      
 

Contact

The Project

The Human Protein Atlas

The Human Protein Atlas project is funded
by the Knut & Alice Wallenberg foundation.