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Immunofluorescent staining of human cell line U-2 OS shows localization to cytosol & actin filaments.
Immunofluorescent staining of human cell line U-2 OS shows localization to cytosol & actin filaments.
Antibody dilution
1:50
1:125
Literature conformity
The subcellular location is partly supported by literature or no literature is available.
The subcellular location is supported by literature.
IMMUNOHISTOCHEMISTRY
Antibody HPA034607
Antibody CAB047325
Antibody CAB047326
Standard validation
Supported
Supported
Figure description
Immunohistochemical staining of human small intestine shows strong cytoplasmic positivity in glandular cells.
Immunohistochemical staining of human small intestine shows strong cytoplasmic and nuclear positivity in glandular cells.
Expression
RNA: detected in 6 tissues Protein: detected in 10 cell types
RNA: detected in 6 tissues Protein: detected in 4 cell types
Retrieval
HIER pH6
HIER pH6
Antibody dilution
1:250
1:200
Literature conformity
Consistent with extensive gene/protein characterization data.
Consistent with extensive gene/protein characterization data.
RNA consistency
Mainly consistent with RNA expression data.
Consistent with RNA expression data.
WESTERN BLOT
Antibody HPA034607
Antibody CAB047325
Antibody CAB047326
Standard validation
Supported
Single band corresponding to the predicted size in kDa (+/-20%).
Uncertain
Analysis performed using a standard panel of samples. Current setup is not applicable due to low RNA count.
Uncertain
Analysis performed using a standard panel of samples. Current setup is not applicable due to low RNA count.
Figure description
Lane 1: Marker [kDa] 250, 130, 95, 72, 55, 36, 28, 17, 10 Lane 2: Negative control (vector only transfected HEK293T lysate) Lane 3: Over-expression Lysate (Co-expressed with a C-terminal myc-DDK tag (~3.1 kDa) in mammalian HEK293T cells, LY424906)
Target mass (kDa)
15.2
15.2
15.2
Antibody dilution
1:250
1:500
1:500
PROTEIN ARRAY
Antibody HPA034607
Antibody CAB047325
Antibody CAB047326
Standard validation
Supported
Pass with single peak corresponding to interaction only with its own antigen.
Figure description
Antibody specificity analysis with protein arrays. Predicted and matching interactions are shown in green.
