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SKP2

ANTIBODY INFORMATION
 
 

Antibody HPA051196

  

Antibody HPA054633

  

Antibody CAB013491

  

Antibody CAB013533

  

Provider

 Atlas Antibodies
Sigma-Aldrich
   Atlas Antibodies
Sigma-Aldrich
   Zymed
   Zymed
  

Product name

 HPA051196   HPA054633   32-3300   18-0307  

Host species

 Rabbit   Rabbit   Mouse   Mouse  

Clonality

 pAb   pAb   mAb   mAb  

Purity

 Affinity purified using the PrEST-antigen as affinity ligand   Affinity purified using the PrEST-antigen as affinity ligand   Not known   Not known  

Other gene match

        

Released in version

 12   14   14   4  

References

        

VALIDATION SUMMARY
 
 

Antibody HPA051196

  

Antibody HPA054633

  

Antibody CAB013491

  

Antibody CAB013533

  
Immunocytochemistry

Supported        
Immunohistochemistry

  Approved   Approved   Approved  
Western blot

Supported   Supported   Uncertain   Supported  
Protein array

Supported   Supported      

IMMUNOCYTOCHEMISTRY
 
 

Antibody HPA051196

  

Antibody HPA054633

  

Antibody CAB013491

  

Antibody CAB013533

  

Standard validation

 Supported        

       

Figure description

 Immunofluorescent staining of human cell line PC-3 shows localization to nucleus, nucleoli & cytosol.        

Antibody dilution

 1:37        

Literature conformity

 The subcellular location is supported by literature.        

IMMUNOHISTOCHEMISTRY
 
 

Antibody HPA051196

  

Antibody HPA054633

  

Antibody CAB013491

  

Antibody CAB013533

  

Standard validation

   Approved   Approved   Approved  

       

Figure description

   Immunohistochemical staining of human placenta shows cytoplasmic positivity in trophoblastic cells.   Immunohistochemical staining of human placenta shows nuclear positivity in trophoblastic cells.   Immunohistochemical staining of human placenta shows strong nuclear positivity in a subset of trophoblastic cells.  

Expression

   RNA: detected in 36 tissues
Protein: detected in 61 cell types		   RNA: detected in 36 tissues
Protein: detected in 28 cell types		   RNA: detected in 36 tissues
Protein: detected in 54 cell types		  

Retrieval

   HIER pH6   HIER pH6   HIER pH6  

Antibody dilution

   1:50   1:1500   1:20  

Literature conformity

   Partly consistent with extensive gene/protein characterization data.   Partly consistent with extensive gene/protein characterization data.   Partly consistent with extensive gene/protein characterization data.  

RNA consistency

   Mainly not consistent with RNA expression data.   Mainly not consistent with RNA expression data.   Mainly not consistent with RNA expression data.  

WESTERN BLOT
 
 

Antibody HPA051196

  

Antibody HPA054633

  

Antibody CAB013491

  

Antibody CAB013533

  

Standard validation

 Supported

Analysis performed using a standard panel of samples.
Band of predicted size in kDa (+/-20%) with additional bands present.		   Supported

Analysis performed using a standard panel of samples.
Single band corresponding to the predicted size in kDa (+/-20%).		   Uncertain

Analysis performed using a standard panel of samples.
Weak band of predicted size but with additional bands of higher intensity also present.		   Supported

Analysis performed using a standard panel of samples.
Single band corresponding to the predicted size in kDa (+/-20%).		  

       

Figure description

 Lane 1: Marker [kDa] 250, 130, 95, 72, 55, 36, 28, 17, 10
Lane 2: RT4
Lane 3: U-251 MG
Lane 4: Human Plasma
Lane 5: Liver
Lane 6: Tonsil		   Lane 1: Marker [kDa] 250, 130, 95, 72, 55, 36, 28, 17, 10
Lane 2: RT4
Lane 3: U-251 MG
Lane 4: Human Plasma
Lane 5: Liver
Lane 6: Tonsil		     Lane 1: Marker [kDa] 213, 118, 83, 50, 33, 26, 18
Lane 2: RT4
Lane 3: U-251 MG
Lane 4: Human Plasma
Lane 5: Liver
Lane 6: Tonsil		  

Target mass (kDa)

 48.9, 47.8, 24.7, 23.8   48.9, 47.8, 46.6   48.9, 47.8, 46.6, 28.6, 24.7, 23.8   48.9, 47.8, 46.6, 28.6, 24.7, 23.8  

Antibody dilution

 1:190   1:190   1:500   1:500  

PROTEIN ARRAY
 
 

Antibody HPA051196

  

Antibody HPA054633

  

Antibody CAB013491

  

Antibody CAB013533

  

Standard validation

 Supported

Pass with single peak corresponding to interaction only with its own antigen.		   Supported

Pass with single peak corresponding to interaction only with its own antigen.		      

       

Figure description

 Antibody specificity analysis with protein arrays. Predicted and matching interactions are shown in green.   Antibody specificity analysis with protein arrays. Predicted and matching interactions are shown in green.      

Antibody dilution

 1:1500   1:1500      

ANTIGEN INFORMATION
 
 

Antibody HPA051196

  

Antibody HPA054633

  

Antibody CAB013491

  

Antibody CAB013533

  

Antigen

 Recombinant protein fragment   Recombinant protein fragment   Not known   Not known  

Length (aa)

 84   83      

Antigen sequence

 
SLSRCYDIIPETLLELGEIPTLKTLQVFGIVPDGTLQLLKEALPHLQINC
SHFTTIARPTIGNKKNQEIWGIKCRLTLQKPSCL
  
LASDESLWQTLDLTGKNLHPDVTGRLLSQGVIAFRCPRSFMDQPLAEHFS
PFRVQHMDLSNSVIEVSTLHGILSQCSKLQNLS
  

											 
																						

											 
																				

																		

										
											
Matching transcripts

												
																					SKP2-002 - ENSP00000274255 [100%] 
SKP2-006 - ENSP00000421941 [100%] 
SKP2-201 - ENSP00000443492 [100%] 
SKP2-202 - ENSP00000478031 [100%] 

											 
																						SKP2-001 - ENSP00000274254 [100%] 
SKP2-002 - ENSP00000274255 [100%] 
SKP2-201 - ENSP00000443492 [100%] 

											 
																						
											 
																						
											 
																				

																		

										
											
Other gene match

												
																					
											 
																						
											 
																						
											 
																						
											 
																				

																		

										ANTIGEN VIEW
									
									
										

		
			

				

										
						

							

								

									
SKP2-001
SKP2-002
SKP2-006
SKP2-201
SKP2-202
								

							

						

							
									

			

				
	

	

		
					
	

													
															
				
						

				 
			

		

			
	











	

		


			

				

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