RNF145 - Antibodies - The Human Protein Atlas

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RNF145

TISSUE

CELL

CANCER

ANTIBODY INFORMATION

Antibody HPA036562

Provider

Atlas Antibodies
Sigma-Aldrich

Product name

HPA036562

Host species

Rabbit

Clonality

pAb

Purity

Affinity purified using the PrEST-antigen as affinity ligand

Other gene match

Released in version

References

VALIDATION SUMMARY

Antibody HPA036562

Immunocytochemistry

Immunohistochemistry

Western blot

Protein array

IMMUNOCYTOCHEMISTRY

Antibody HPA036562

Standard validation

Approved

Figure description

Immunofluorescent staining of human cell line U-2 OS shows localization to nucleoplasm, plasma membrane & cytosol.

Antibody dilution

1:98

Literature conformity

The subcellular location is partly supported by literature or no literature is available.

IMMUNOHISTOCHEMISTRY

Antibody HPA036562

Standard validation

Uncertain

Figure description

Immunohistochemical staining of human skin shows strong cytoplasmic positivity in epidermal cells.

Expression

RNA: detected in 37 tissues
Protein: detected in 50 cell types

Retrieval

HIER pH6

Antibody dilution

1:700

Literature conformity

No avaliable gene/protein characterization data.

RNA consistency

Mainly not consistent with RNA expression data.

WESTERN BLOT

Antibody HPA036562

Standard validation

Uncertain

Analysis performed using a standard panel of samples.
Weak band of predicted size but with additional bands of higher intensity also present.

Figure description

Target mass (kDa)

79.2, 78.9, 77.6, 77.3, 75.6

Antibody dilution

1:250

PROTEIN ARRAY

Antibody HPA036562

Standard validation

Approved

Pass with quality comment low specificity (binding to 1-2 PrESTs >15% and <40%).

Figure description

Antibody specificity analysis with protein arrays. Predicted and matching interactions are shown in green.

Antibody dilution

1:3000

ANTIGEN INFORMATION

Antibody HPA036562

Antigen

Recombinant protein fragment

Length (aa)

Antigen sequence

NSSQLPGLGTEPVLQPHAGAEQNVMFQEGTEPPGQEHTPGTRIQEGSRDN
NEYIARRPDNQEGAFDPKEYPHSAKDEAHPVESA

Matching transcripts

RNF145-001 - ENSP00000274542 [100%]
RNF145-002 - ENSP00000409064 [100%]
RNF145-003 - ENSP00000430955 [100%]
RNF145-004 - ENSP00000429071 [100%]
RNF145-005 - ENSP00000430397 [100%]
RNF145-006 - ENSP00000430753 [100%]
RNF145-201 - ENSP00000482720 [100%]

Other gene match

ANTIGEN VIEW

RNF145-001	RNF145-002	RNF145-003	RNF145-004	RNF145-005	RNF145-006	RNF145-201

Contact

contact@proteinatlas.org

The Project

The Human Protein Atlas

The Human Protein Atlas project is funded
by the Knut & Alice Wallenberg foundation.