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Immunofluorescent staining of human cell line RH-30 shows localization to nucleoplasm.
Immunofluorescent staining of human cell line MCF7 shows localization to nucleus, centrosome & cell junctions.
Antibody dilution
1:200
1:53
Literature conformity
The subcellular location is not consistent with literature.
The subcellular location is not consistent with literature.
IMMUNOHISTOCHEMISTRY
Antibody HPA030972
Antibody HPA030973
Antibody HPA061387
Standard validation
Uncertain
Uncertain
Figure description
Immunohistochemical staining of human kidney shows strong cytoplasmic positivity in granular pattern in cells in tubules.
Immunohistochemical staining of human kidney shows strong granular positivity in tubular cells.
Expression
RNA: detected in 26 tissues Protein: detected in 78 cell types
RNA: detected in 26 tissues Protein: detected in 68 cell types
Retrieval
HIER pH6
HIER pH6
Antibody dilution
1:200
1:125
Literature conformity
No avaliable gene/protein characterization data.
No avaliable gene/protein characterization data.
RNA consistency
Mainly not consistent with RNA expression data.
Mainly consistent with RNA expression data.
WESTERN BLOT
Antibody HPA030972
Antibody HPA030973
Antibody HPA061387
Standard validation
Supported
Band of predicted size in kDa (+/-20%) with additional bands present.
Supported
Single band corresponding to the predicted size in kDa (+/-20%).
Supported
Analysis performed using a standard panel of samples. Band of predicted size in kDa (+/-20%) with additional bands present.
Figure description
Lane 1: Marker [kDa] 250, 130, 95, 72, 55, 36, 28, 17, 10 Lane 2: Negative control (vector only transfected HEK293T lysate) Lane 3: Over-expression Lysate (Co-expressed with a C-terminal myc-DDK tag (~3.1 kDa) in mammalian HEK293T cells, LY414797)
Lane 1: Marker [kDa] 250, 130, 95, 72, 55, 36, 28, 17, 10 Lane 2: Negative control (vector only transfected HEK293T lysate) Lane 3: Over-expression Lysate (Co-expressed with a C-terminal myc-DDK tag (~3.1 kDa) in mammalian HEK293T cells, LY414797)
Lane 1: Marker [kDa] 250, 130, 95, 72, 55, 36, 28, 17, 10 Lane 2: RT4 Lane 3: U-251 MG Lane 4: Human Plasma Lane 5: Liver Lane 6: Tonsil
Target mass (kDa)
60.5
60.5
60.5
Antibody dilution
1:250
1:250
1:180
PROTEIN ARRAY
Antibody HPA030972
Antibody HPA030973
Antibody HPA061387
Standard validation
Supported
Pass with single peak corresponding to interaction only with its own antigen.
Supported
Pass with single peak corresponding to interaction only with its own antigen.
Approved
Pass with quality comment low specificity (binding to 1-2 PrESTs >15% and <40%).
Figure description
Antibody specificity analysis with protein arrays. Predicted and matching interactions are shown in green.
Antibody specificity analysis with protein arrays. Predicted and matching interactions are shown in green.
Antibody specificity analysis with protein arrays. Predicted and matching interactions are shown in green.
