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Immunofluorescent staining of human cell line U-2 OS shows localization to nucleus, nucleoli fibrillar center & cytosol.
Immunofluorescent staining of human cell line U-2 OS shows localization to nucleus, nucleoli fibrillar center & cytosol.
Antibody dilution
1:74
1:10
Literature conformity
The subcellular location is supported by literature.
The subcellular location is supported by literature.
IMMUNOHISTOCHEMISTRY
Antibody HPA002692
Antibody HPA005941
Antibody CAB010272
Standard validation
Supported
Supported
Supported
Figure description
Immunohistochemical staining of human tonsil shows moderate cytoplasmic positivity.
Immunohistochemical staining of human lymph node shows strong cytoplasmic positivity in reaction center cells and lymphoid cells outside reaction center.
Immunohistochemical staining of human tonsil shows strong positivity.
Expression
RNA: detected in 36 tissues Protein: detected in 46 cell types
RNA: detected in 36 tissues Protein: detected in 75 cell types
RNA: detected in 36 tissues Protein: detected in 79 cell types
Retrieval
HIER pH6
HIER pH6
HIER pH6
Antibody dilution
1:100
1:60
1:40
Literature conformity
Partly consistent with gene/protein characterization data.
Consistent with gene/protein characterization data.
Consistent with gene/protein characterization data.
RNA consistency
Mainly not consistent with RNA expression data.
Mainly consistent with RNA expression data.
Mainly consistent with RNA expression data.
WESTERN BLOT
Antibody HPA002692
Antibody HPA005941
Antibody CAB010272
Standard validation
Uncertain
Analysis performed using a standard panel of samples. Weak band of predicted size but with additional bands of higher intensity also present.
Supported
Analysis performed using a standard panel of samples. Single band corresponding to the predicted size in kDa (+/-20%).
Supported
Analysis performed using a standard panel of samples. Band of predicted size in kDa (+/-20%) with additional bands present.
Figure description
Lane 1: Marker [kDa] 220, 112, 84, 47, 32, 26, 16.8 Lane 2: RT4 Lane 3: U-251 MG Lane 4: Human Plasma Lane 5: Liver Lane 6: Tonsil
Lane 1: Marker [kDa] 229, 107, 78, 46, 31, 26, 16.2 Lane 2: RT4 Lane 3: U-251 MG Lane 4: Human Plasma Lane 5: Liver Lane 6: Tonsil
Target mass (kDa)
52.9, 52.7, 38.5
52.9, 52.7, 38.5
52.9, 52.7, 38.5, 5.9
Antibody dilution
1:250
1:250
1:500
PROTEIN ARRAY
Antibody HPA002692
Antibody HPA005941
Antibody CAB010272
Standard validation
Supported
Pass with single peak corresponding to interaction only with its own antigen.
Approved
Pass with quality comment low specificity (binding to 1-2 PrESTs >15% and <40%).
Figure description
Antibody specificity analysis with protein arrays. Predicted and matching interactions are shown in green.
Antibody specificity analysis with protein arrays. Predicted and matching interactions are shown in green.
