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Immunofluorescent staining of human cell line SH-SY5Y shows localization to endoplasmic reticulum.
Antibody dilution
1:76
Literature conformity
The subcellular location is partly supported by literature or no literature is available.
IMMUNOHISTOCHEMISTRY
Antibody HPA017324
Antibody HPA072701
Antibody CAB018649
Standard validation
Supported
Supported
Figure description
Immunohistochemical staining of human pancreas shows distinct cytoplasmic positivity in islet cells.
Immunohistochemical staining of human pancreas shows strong cytoplasmic positivity in islet cells.
Expression
RNA: detected in 19 tissues Protein: detected in 38 cell types
RNA: detected in 19 tissues Protein: detected in 50 cell types
Retrieval
HIER pH6
HIER pH6
Antibody dilution
1:20
1:200
Literature conformity
Partly consistent with extensive gene/protein characterization data.
Partly consistent with extensive gene/protein characterization data.
RNA consistency
Mainly not consistent with RNA expression data.
Mainly not consistent with RNA expression data.
WESTERN BLOT
Antibody HPA017324
Antibody HPA072701
Antibody CAB018649
Standard validation
Uncertain
No bands detected.
Uncertain
Analysis performed using a standard panel of samples. Weak band of predicted size but with additional bands of higher intensity also present.
Figure description
Lane 1: Marker [kDa] 250, 130, 95, 72, 55, 36, 28, 17, 10 Lane 2: Negative control (vector only transfected HEK293T lysate) Lane 3: Over-expression Lysate (Co-expressed with a C-terminal myc-DDK tag (~3.1 kDa) in mammalian HEK293T cells, LY405128)
Target mass (kDa)
19.2, 19.1, 13.4
19.2, 19.1, 13.4
Antibody dilution
1:250
1:500
PROTEIN ARRAY
Antibody HPA017324
Antibody HPA072701
Antibody CAB018649
Standard validation
Approved
Pass with quality comment low specificity (binding to 1-2 PrESTs >15% and <40%).
Supported
Pass with single peak corresponding to interaction only with its own antigen.
Figure description
Antibody specificity analysis with protein arrays. Predicted and matching interactions are shown in green.
Antibody specificity analysis with protein arrays. Predicted and matching interactions are shown in green.
