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EGFR

ANTIBODY INFORMATION
 
 

Antibody HPA001200

  

Antibody HPA018530

  

Antibody CAB000035

  

Antibody CAB068186

  

Antibody CAB073534

  

Provider

 Atlas Antibodies
Sigma-Aldrich
   Atlas Antibodies
Sigma-Aldrich
   Zymed
   Atlas Antibodies
Sigma-Aldrich
   Monash University MATF
  

Product name

 HPA001200   HPA018530   28-0005   AMAb90816   HUPO 5 (anti-EGFR)  

Host species

 Rabbit   Rabbit   Mouse   Mouse   Mouse  

Clonality

 pAb   pAb   mAb   mAb   mAb  

Purity

 Affinity purified using the PrEST-antigen as affinity ligand   Affinity purified using the PrEST-antigen as affinity ligand   Not known   Protein A/G   Protein A/G  

Other gene match

          

Released in version

 3   4   1   13   14  

References

 1   2        

VALIDATION SUMMARY
 
 

Antibody HPA001200

  

Antibody HPA018530

  

Antibody CAB000035

  

Antibody CAB068186

  

Antibody CAB073534

  
Immunocytochemistry

  Supported   Supported      
Immunohistochemistry


Validated
   Supported   Supported  

Validated
   Supported  
Western blot

Uncertain   Uncertain   Supported   Supported   Uncertain  
Protein array

Supported   Supported        

IMMUNOCYTOCHEMISTRY
 
 

Antibody HPA001200

  

Antibody HPA018530

  

Antibody CAB000035

  

Antibody CAB068186

  

Antibody CAB073534

  

Standard validation

   Supported   Supported      

         

Figure description

   Immunofluorescent staining of human cell line A-431 shows localization to plasma membrane.   Immunofluorescent staining of human cell line A-431 shows localization to plasma membrane.      

Antibody dilution

   1:56   1:10      

Literature conformity

   The subcellular location is supported by literature.   The subcellular location is supported by literature.      

IMMUNOHISTOCHEMISTRY
 
 

Antibody HPA001200

  

Antibody HPA018530

  

Antibody CAB000035

  

Antibody CAB068186

  

Antibody CAB073534

  

Formal validation: Orthogonal
Validated

Pearson correlation >0.6 for protein and RNA expression in cell lines.		      
Validated

Pearson correlation >0.6 for protein and RNA expression in cell lines.		    

 

 

      
 

 

    

Figure description

 Distribution of protein expression (antibody staining) and RNA expression (TPM) across 44 cell lines.       Distribution of protein expression (antibody staining) and RNA expression (TPM) across 44 cell lines.    
 

Standard validation

 Supported   Supported   Supported   Supported   Supported  

         

Figure description

 Immunohistochemical staining of human placenta shows strong cytoplasmic and membranous positivity in trophoblastic cells.   Immunohistochemical staining of human placenta shows strong cytoplasmic positivity in trophoblastic cells.   Immunohistochemical staining of human placenta shows membranous positivity in trophoblastic cells.   Immunohistochemical staining of human placenta shows distinct membranous positivity in trophoblastic cells.   Immunohistochemical staining of human placenta shows strong membranous positivity in trophoblastic cells.  

Expression

 RNA: detected in 36 tissues
Protein: detected in 8 cell types		   RNA: detected in 36 tissues
Protein: detected in 53 cell types		   RNA: detected in 36 tissues
Protein: detected in 46 cell types		   RNA: detected in 36 tissues
Protein: detected in 7 cell types		   RNA: detected in 36 tissues
Protein: detected in 39 cell types		  

Retrieval

 HIER pH6   HIER pH6   Proteinase K.   HIER pH6   HIER pH6  

Antibody dilution

 1:25   1:100   1:40   1:2000   1:250000  

Literature conformity

 Partly consistent with extensive gene/protein characterization data.   Consistent with extensive gene/protein characterization data.   Partly consistent with extensive gene/protein characterization data.   Partly consistent with extensive gene/protein characterization data.   Consistent with extensive gene/protein characterization data.  

RNA consistency

 Mainly consistent with RNA expression data.   Mainly consistent with RNA expression data.   Mainly consistent with RNA expression data.   Mainly consistent with RNA expression data.   Mainly consistent with RNA expression data.  

WESTERN BLOT
 
 

Antibody HPA001200

  

Antibody HPA018530

  

Antibody CAB000035

  

Antibody CAB068186

  

Antibody CAB073534

  

Standard validation

 Uncertain

Analysis performed using a standard panel of samples.
Only bands not corresponding to the predicted size.		   Uncertain

Analysis performed using a standard panel of samples.
Only bands not corresponding to the predicted size.		   Supported

Analysis performed using a standard panel of samples.
Band of predicted size in kDa (+/-20%) with additional bands present.		   Supported

Analysis performed using a standard panel of samples.
Band of predicted size in kDa (+/-20%) with additional bands present.		   Uncertain

Analysis performed using a standard panel of samples.
Weak band of predicted size but with additional bands of higher intensity also present.		  

         

Figure description

     Lane 1: Marker [kDa] 219, 112, 85, 49, 32, 25, 17.5
Lane 2: RT4
Lane 3: U-251 MG
Lane 4: A-431
Lane 5: Liver
Lane 6: Tonsil		   Lane 1: Marker [kDa] 250, 130, 100, 70, 55, 35, 25, 15, 10
Lane 2: RT4
Lane 3: U-251 MG
Lane 4: Human Plasma
Lane 5: Liver
Lane 6: Tonsil		    

Target mass (kDa)

 134.3, 129.2, 120.7, 77.3, 72.4, 69.2   134.3, 129.2   134.3, 129.2, 120.7, 77.3, 72.4, 69.2, 44.7, 14.7   134.3, 129.2, 120.7, 77.3, 72.4, 69.2, 44.7, 14.7   134.3, 129.2, 120.7, 77.3, 72.4, 69.2, 44.7, 14.7  

Antibody dilution

 1:250   1:250   1:400   1:1000   1:1000  

PROTEIN ARRAY
 
 

Antibody HPA001200

  

Antibody HPA018530

  

Antibody CAB000035

  

Antibody CAB068186

  

Antibody CAB073534

  

Standard validation

 Supported

Pass with single peak corresponding to interaction only with its own antigen.		   Supported

Pass with single peak corresponding to interaction only with its own antigen.		        

         

Figure description

 Antibody specificity analysis with protein arrays. Predicted and matching interactions are shown in green.   Antibody specificity analysis with protein arrays. Predicted and matching interactions are shown in green.        

Antibody dilution

 1:3000   1:3000        

ANTIGEN INFORMATION
 
 

Antibody HPA001200

  

Antibody HPA018530

  

Antibody CAB000035

  

Antibody CAB068186

  

Antibody CAB073534

  

Antigen

 Recombinant protein fragment   Recombinant protein fragment   Not known   Recombinant protein   Not known  

Length (aa)

 149   149        

Antigen sequence

 
EFKDSLSINATNIKHFKNCTSISGDLHILPVAFRGDSFTHTPPLDPQELD
ILKTVKEITGFLLIQAWPENRTDLHAFENLEIIRGRTKQHGQFSLAVVSL
NITSLGLRSLKEISDGDVIISGNKNLCYANTINWKKLFGTSGQKTKIIS
  
QQGFFSSPSTSRTPLLSSLSATSNNSTVACIDRNGLQSCPIKEDSFLQRY
SSDPTGALTEDSIDDTFLPVPEYINQSVPKRPAGSVQNPVYHNQPLNPAP
SRDPHYQDPHSTAVGNPEYLNTVQPTCVNSTFDSPAHWAQKGSHQISLD
  

											 
																						

											 
																						

											 
																				

																		

										
											
Matching transcripts

												
																					EGFR-001 - ENSP00000275493 [100%] 
EGFR-002 - ENSP00000345973 [100%] 
EGFR-003 - ENSP00000342376 [100%] 
EGFR-004 - ENSP00000415559 [100%] 
EGFR-201 - ENSP00000410031 [100%] 
EGFR-202 - ENSP00000395243 [100%] 

											 
																						EGFR-001 - ENSP00000275493 [100%] 
EGFR-202 - ENSP00000395243 [100%] 

											 
																						
											 
																						
											 
																						
											 
																				

																		

										
											
Other gene match

												
																					
											 
																						
											 
																						
											 
																						
											 
																						
											 
																				

																		

										ANTIGEN VIEW
									
									
										

		
			

				

										
						

							

								

									
EGFR-001
EGFR-002
EGFR-003
EGFR-004
EGFR-201
EGFR-202
								

							

						

							
									

			

				
	

	

		
					
	

													
															
				
											

									
										
										
										
										
RELEVANT PUBLICATIONS

											
								

								

									 
								

								

									 
																			
											
											
											
Antibody HPA001200

												
										 
																				
											
											
											
Antibody HPA018530

												
										 
																				
											
											
											
Antibody CAB000035

												
										 
																				
											
											
											
Antibody CAB068186

												
										 
																				
											
											
											
Antibody CAB073534

												
										 
																		

																	

										
											
1.

												
																						Multiplex epitope mapping using bacterial surface display reveals both linear and conformational epitopes

	Hudson EP et al

	Sci Rep 2012;2:706

			
											 
																							Proteomic screen reveals Fbw7 as a modulator of the NF-κB pathway

	Arabi A et al

	Nat Commun 2012;3:976

			
											 
																						
											 
																						
											 
																						
											 
																				

																		

										
											
2.

												
																					
											 
																							Sentinel lymph node biopsy revisited: ultrasound-guided photoacoustic detection of micrometastases using molecularly targeted plasmonic nanosensors

	Luke GP et al

	Cancer Res 2014;74(19):5397-408

			Application: IHC				
											 
																						
											 
																						
											 
																						
											 
																				

															
				
						

				 
			

		

			
	











	

		


			

				

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