IGFBP1 - Antibodies - The Human Protein Atlas

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IGFBP1

TISSUE

CELL

CANCER

ANTIBODY INFORMATION

Antibody HPA046972

Antibody HPA050640

Antibody CAB004445

Provider

Atlas Antibodies
Sigma-Aldrich

Santa Cruz Biotechnology

Product name

HPA046972

HPA050640

sc-25257

Host species

Rabbit

Mouse

Clonality

pAb

mAb

Purity

Affinity purified using the PrEST-antigen as affinity ligand

Protein A/G

Other gene match

Released in version

References

VALIDATION SUMMARY

Antibody HPA046972

Antibody HPA050640

Antibody CAB004445

Immunocytochemistry

Immunohistochemistry

Western blot

Protein array

IMMUNOCYTOCHEMISTRY

Antibody HPA046972

Antibody HPA050640

Antibody CAB004445

Standard validation

Supported

Figure description

Immunofluorescent staining of human cell line Hep G2 shows localization to the Golgi apparatus.

Antibody dilution

1:40

Literature conformity

The subcellular location is supported by literature.

IMMUNOHISTOCHEMISTRY

Antibody HPA046972

Antibody HPA050640

Antibody CAB004445

Formal validation: Independent

Validated

Pearson correlation >0.6 for protein expression in cell lines using independent antibodies.

Validated

Pearson correlation >0.6 for protein expression in cell lines using independent antibodies.

Figure description

Distribution of protein expression (antibody staining).
Pearson correlation with HPA050640 across 46 cell lines.

Distribution of protein expression (antibody staining).
Pearson correlation with HPA046972 across 46 cell lines.

Formal validation: Independent

Validated

Spearman correlation >0.6 for protein expression in tissues using independent antibodies.

Validated

Spearman correlation >0.6 for protein expression in tissues using independent antibodies.

Figure description

Distribution of protein expression (antibody staining).
Spearman correlation with HPA050640 across 76 cell types.

Distribution of protein expression (antibody staining).
Spearman correlation with HPA046972 across 76 cell types.

Standard validation

Supported

Figure description

Immunohistochemical staining of human placenta shows moderate cytoplasmic positivity in decidual cells.

Immunohistochemical staining of human placenta shows strong cytoplasmic positivity in decidual cells.

Expression

RNA: detected in 7 tissues
Protein: detected in 2 cell types

RNA: detected in 7 tissues
Protein: detected in 4 cell types

RNA: detected in 7 tissues
Protein: detected in 3 cell types

Retrieval

HIER pH6

Antibody dilution

1:3000

1:7000

1:10000

Literature conformity

Consistent with extensive gene/protein characterization data.

RNA consistency

Mainly consistent with RNA expression data.

WESTERN BLOT

Antibody HPA046972

Antibody HPA050640

Antibody CAB004445

Standard validation

Uncertain

Analysis performed using a standard panel of samples.
No bands detected.

Supported

Analysis performed using a standard panel of samples.
Single band corresponding to the predicted size in kDa (+/-20%).

Figure description

Lane 1: Marker [kDa] 250, 130, 95, 72, 55, 36, 28, 17, 10
Lane 2: RT4
Lane 3: U-251 MG
Lane 4: Human Plasma
Lane 5: Liver
Lane 6: Tonsil

Lane 1: Marker [kDa] 220, 112, 84, 47, 32, 26, 16.8
Lane 2: RT4
Lane 3: U-251 MG
Lane 4: Human Plasma
Lane 5: Liver
Lane 6: Tonsil

Target mass (kDa)

27.9, 27.7, 22.9

27.9, 27.7

27.9, 27.7, 22.9

Antibody dilution

1:200

1:360

1:500

PROTEIN ARRAY

Antibody HPA046972

Antibody HPA050640

Antibody CAB004445

Standard validation

Supported

Pass with single peak corresponding to interaction only with its own antigen.

Approved

Pass with quality comment low specificity (binding to 1-2 PrESTs >15% and <40%).

Figure description

Antibody specificity analysis with protein arrays. Predicted and matching interactions are shown in green.

Antibody dilution

1:1600

1:2850

ANTIGEN INFORMATION

Antibody HPA046972

Antibody HPA050640

Antibody CAB004445

Antigen

Recombinant protein fragment

Recombinant protein

Length (aa)

Antigen sequence

QQPLHALTRGQGACVQESDASAPHAAEAGSPESPESTEITEEELLDNFHL
MAPSEEDHSILWDAISTYDGS

SKALHVTNIKKWKEPCRIELYRVVESLAKAQETSGEEISKFYLPNCNKNG
FYHSRQCETSMDGEAGLCWCVYPWNGKRIPGSPEIRGDPNCQI

Matching transcripts

IGFBP1-001 - ENSP00000275525 [100%]
IGFBP1-002 - ENSP00000413511 [100%]
IGFBP1-003 - ENSP00000417069 [100%]

IGFBP1-001 - ENSP00000275525 [100%]
IGFBP1-002 - ENSP00000413511 [98%]

Other gene match

ANTIGEN VIEW

IGFBP1-001	IGFBP1-002	IGFBP1-003

RELEVANT PUBLICATIONS

Antibody HPA046972

Antibody HPA050640

Antibody CAB004445

Affinity proteomics reveals elevated muscle proteins in plasma of children with cerebral malaria
Bachmann J et al
PLoS Pathog 2014;10(4):e1004038

Contact

contact@proteinatlas.org

The Project

The Human Protein Atlas

The Human Protein Atlas project is funded
by the Knut & Alice Wallenberg foundation.