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CXorf57

ANTIBODY INFORMATION
 
 

Antibody HPA000896

  

Antibody HPA001374

  

Provider

 Atlas Antibodies
Sigma-Aldrich
   Atlas Antibodies
Sigma-Aldrich
  

Product name

 HPA000896   HPA001374  

Host species

 Rabbit   Rabbit  

Clonality

 pAb   pAb  

Purity

 Affinity purified using the PrEST-antigen as affinity ligand   Affinity purified using the PrEST-antigen as affinity ligand  

Other gene match

    

Released in version

 1   10  

References

    

VALIDATION SUMMARY
 
 

Antibody HPA000896

  

Antibody HPA001374

  
Immunocytochemistry

  Approved  
Immunohistochemistry

Uncertain   Uncertain  
Western blot

Uncertain   Uncertain  
Protein array

Supported   Approved  

IMMUNOCYTOCHEMISTRY
 
 

Antibody HPA000896

  

Antibody HPA001374

  

Standard validation

   Approved  

   

Figure description

   Immunofluorescent staining of human cell line A-431 shows localization to intermediate filaments.  

Antibody dilution

   1:8  

Literature conformity

   The subcellular location is partly supported by literature or no literature is available.  

IMMUNOHISTOCHEMISTRY
 
 

Antibody HPA000896

  

Antibody HPA001374

  

Standard validation

 Uncertain   Uncertain  

   

Figure description

 Immunohistochemical staining of human rectum shows strong cytoplasmic positivity in glandular cells.   Immunohistochemical staining of human placenta shows distinct membranous positivity in trophoblasts.  

Expression

 RNA: detected in 27 tissues
Protein: detected in 75 cell types		   RNA: detected in 27 tissues
Protein: detected in 32 cell types		  

Retrieval

 HIER pH6   HIER pH6  

Antibody dilution

 1:20   1:100  

Literature conformity

 No avaliable gene/protein characterization data.   No avaliable gene/protein characterization data.  

RNA consistency

 Mainly not consistent with RNA expression data.   Mainly not consistent with RNA expression data.  

WESTERN BLOT
 
 

Antibody HPA000896

  

Antibody HPA001374

  

Standard validation

 Uncertain

Analysis performed using a standard panel of samples.
Weak band of predicted size but with additional bands of higher intensity also present.		   Uncertain

Analysis performed using a standard panel of samples.
Weak band of predicted size but with additional bands of higher intensity also present.		  

   

Figure description

    

Target mass (kDa)

 97.6, 86.6, 65.1   97.6, 86.6, 65.1  

Antibody dilution

 1:500   1:250  

PROTEIN ARRAY
 
 

Antibody HPA000896

  

Antibody HPA001374

  

Standard validation

 Supported

Pass with single peak corresponding to interaction only with its own antigen.		   Approved

Pass with quality comment low specificity (binding to 1-2 PrESTs >15% and <40%).		  

   

Figure description

 Antibody specificity analysis with protein arrays. Predicted and matching interactions are shown in green.   Antibody specificity analysis with protein arrays. Predicted and matching interactions are shown in green.  

Antibody dilution

 1:1000   1:2000  

ANTIGEN INFORMATION
 
 

Antibody HPA000896

  

Antibody HPA001374

  

Antigen

 Recombinant protein fragment   Recombinant protein fragment  

Length (aa)

 117   111  

Antigen sequence

 
TISDRWESQLWREKKFGLIDHLHYSRVYPESIPRKFMFEHRKFLSDQYNS
QPAKYVPPEGRPPKLDDFKSARSLGHFEVTILGLNHEIAIDVAFLPMYCP
EDIRTSQIDTLLTSMNY
  
KQVKPEWRLPKLNHRFTTRSELDDMPENCICDVIGLLVFVGRVQRSKKKE
NREDFWSYRWIHIADGTSEQPFIVELFSTSQPEIFENIYPMAYFVCTQLK
VVRNDNQVPKL
 

Matching transcripts

 CXorf57-002 - ENSP00000361628 [100%]
CXorf57-003 - ENSP00000405866 [100%]
CXorf57-201 - ENSP00000361623 [100%]
  CXorf57-002 - ENSP00000361628 [100%]
CXorf57-003 - ENSP00000405866 [100%]
CXorf57-201 - ENSP00000361623 [100%]
 

Other gene match

    
ANTIGEN VIEW
CXorf57-002
CXorf57-003
CXorf57-201
 

Contact

The Project

The Human Protein Atlas

The Human Protein Atlas project is funded
by the Knut & Alice Wallenberg foundation.