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ANTIBODY INFORMATION
 
 

Antibody HPA000248

  

Antibody HPA000571

  

Provider

 Atlas Antibodies
Sigma-Aldrich
   Atlas Antibodies
Sigma-Aldrich
  

Product name

 HPA000248   HPA000571  

Host species

 Rabbit   Rabbit  

Clonality

 pAb   pAb  

Purity

 Affinity purified using the PrEST-antigen as affinity ligand   Affinity purified using the PrEST-antigen as affinity ligand  

Other gene match

    

Released in version

 1   1  

References

 2   2  

VALIDATION SUMMARY
 
 

Antibody HPA000248

  

Antibody HPA000571

  
Immunocytochemistry


Validated
  

Validated
  
Immunohistochemistry


Validated
  

Validated

Validated
  
Western blot

Supported   Supported  
Protein array

Supported   Supported  

IMMUNOCYTOCHEMISTRY
 
 

Antibody HPA000248

  

Antibody HPA000571

  

Formal validation: Independent
Validated

Antibody staining overlaps with antibody HPA000571.		  
Validated

Antibody staining overlaps with antibody HPA000248.		  
 

Standard validation

 Supported   Supported  

   

Figure description

 Immunofluorescent staining of human cell line A-431 shows localization to endoplasmic reticulum & lipid droplets.   Immunofluorescent staining of human cell line A-431 shows localization to endoplasmic reticulum & lipid droplets.  

Antibody dilution

 1:83   1:30  

Literature conformity

 The subcellular location is supported by literature.   The subcellular location is supported by literature.  

IMMUNOHISTOCHEMISTRY
 
 

Antibody HPA000248

  

Antibody HPA000571

  

Formal validation: Orthogonal

  
Validated

Pearson correlation >0.6 for protein and RNA expression in cell lines.		  

 
 

 

  

Figure description

   Distribution of protein expression (antibody staining) and RNA expression (TPM) across 44 cell lines.  
 

Formal validation: Independent
Validated

Pearson correlation >0.6 for protein expression in cell lines using independent antibodies.		  
Validated

Pearson correlation >0.6 for protein expression in cell lines using independent antibodies.		  

 

  
 

  

Figure description

 Distribution of protein expression (antibody staining).
Pearson correlation with HPA000571 across 46 cell lines.		   Distribution of protein expression (antibody staining).
Pearson correlation with HPA000248 across 46 cell lines.		  
 

Formal validation: Independent
Validated

Spearman correlation >0.6 for protein expression in tissues using independent antibodies.		  
Validated

Spearman correlation >0.6 for protein expression in tissues using independent antibodies.		  

 

  
 

  

Figure description

 Distribution of protein expression (antibody staining).
Spearman correlation with HPA000571 across 72 cell types.		   Distribution of protein expression (antibody staining).
Spearman correlation with HPA000248 across 72 cell types.		  
 

Standard validation

 Supported   Supported  

   

Figure description

 Immunohistochemical staining of human testis shows cytoplasmic positivity in Leydig cells.   Immunohistochemical staining of human adrenal gland shows strong cytoplasmic positivity in cortical cells.  

Expression

 RNA: detected in 37 tissues
Protein: detected in 40 cell types		   RNA: detected in 37 tissues
Protein: detected in 69 cell types		  

Retrieval

 HIER pH9   None  

Antibody dilution

 1:500   1:50  

Literature conformity

 Partly consistent with gene/protein characterization data.   Partly consistent with gene/protein characterization data.  

RNA consistency

 Mainly consistent with RNA expression data.   Mainly consistent with RNA expression data.  

WESTERN BLOT
 
 

Antibody HPA000248

  

Antibody HPA000571

  

Standard validation

 Supported

Analysis performed using a standard panel of samples.
Single band corresponding to the predicted size in kDa (+/-20%).		   Supported

Analysis performed using a standard panel of samples.
Single band corresponding to the predicted size in kDa (+/-20%).		  

   

Figure description

 Lane 1: Marker [kDa] 206, 113, 82, 49, 32, 26, 17.8
Lane 2: RT4
Lane 3: U-251 MG
Lane 4: A-431
Lane 5: Liver
Lane 6: Tonsil		   Lane 1: Marker [kDa] 206, 113, 82, 49, 32, 26, 17.8
Lane 2: RT4
Lane 3: U-251 MG
Lane 4: A-431
Lane 5: Liver
Lane 6: Tonsil		  

Target mass (kDa)

 41.9, 28.1   41.9  

Antibody dilution

 1:250   1:250  

PROTEIN ARRAY
 
 

Antibody HPA000248

  

Antibody HPA000571

  

Standard validation

 Supported

Pass with single peak corresponding to interaction only with its own antigen.		   Supported

Pass with single peak corresponding to interaction only with its own antigen.		  

   

Figure description

 Antibody specificity analysis with protein arrays. Predicted and matching interactions are shown in green.   Antibody specificity analysis with protein arrays. Predicted and matching interactions are shown in green.  

Antibody dilution

 1:2000   1:1000  

ANTIGEN INFORMATION
 
 

Antibody HPA000248

  

Antibody HPA000571

  

Antigen

 Recombinant protein fragment   Recombinant protein fragment  

Length (aa)

 125   121  

Antigen sequence

 
MEPAVSEPMRDQVARTHLTEDTPKVNADIEKVNQNQAKRCTVIGGSGFLG
QHMVEQLLARGYAVNVFDIQQGFDNPQVRFFLGDLCSRQDLYPALKGVNT
VFHCASPPPSSNNKELFYRVNYIGT
  
LPYAMKPIDYYTETKILQERAVLGANDPEKNFLTTAIRPHGIFGPRDPQL
VPILIEAARNGKMKFVIGNGKNLVDFTFVENVVHGHILAAEQLSRDSTLG
GKAFHITNDEPIPFWTFLSRI
 

Matching transcripts

 NSDHL-001 - ENSP00000359297 [100%]
NSDHL-002 - ENSP00000396266 [100%]
NSDHL-201 - ENSP00000391854 [100%]
  NSDHL-001 - ENSP00000359297 [100%]
NSDHL-201 - ENSP00000391854 [100%]
 

Other gene match

    
ANTIGEN VIEW
NSDHL-001
NSDHL-002
NSDHL-201

RELEVANT PUBLICATIONS
 
 

Antibody HPA000248

  

Antibody HPA000571

  

1.

 Immunofluorescence and fluorescent-protein tagging show high correlation for protein localization in mammalian cells
Stadler C et al
Nat Methods 2013;10(4):315-23
   Intercellular transfer of proteins as identified by stable isotope labeling of amino acids in cell culture
Li M et al
J Biol Chem 2010;285(9):6285-97
  

2.

 Proteomic analysis of enriched lysosomes at early phase of camptothecin-induced apoptosis in human U-937 cells
Parent N et al
J Proteomics 2009;72(6):960-73
   Immunofluorescence and fluorescent-protein tagging show high correlation for protein localization in mammalian cells
Stadler C et al
Nat Methods 2013;10(4):315-23
  
 

Contact

The Project

The Human Protein Atlas

The Human Protein Atlas project is funded
by the Knut & Alice Wallenberg foundation.