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Antibody staining overlaps with antibody HPA056837.
Validated
Antibody staining overlaps with antibody HPA017719.
Standard validation
Supported
Supported
Figure description
Immunofluorescent staining of human cell line A549 shows localization to cytosol.
Immunofluorescent staining of human cell line U-2 OS shows localization to cytosol.
Antibody dilution
1:25
1:43
Literature conformity
The subcellular location is supported by literature.
The subcellular location is supported by literature.
IMMUNOHISTOCHEMISTRY
Antibody HPA017719
Antibody HPA056837
Standard validation
Approved
Figure description
Immunohistochemical staining of human tonsil shows distinct cytoplasmic positivity in lymphoid cells.
Expression
RNA: detected in 35 tissues Protein: detected in 15 cell types
Retrieval
HIER pH6
Antibody dilution
1:250
Literature conformity
No avaliable gene/protein characterization data.
RNA consistency
Mainly consistent with RNA expression data.
WESTERN BLOT
Antibody HPA017719
Antibody HPA056837
Standard validation
Uncertain
Analysis performed using a standard panel of samples. Single band differing more than +/-20% from predicted size in kDa and not supported by experimental and/or bioinformatic data.
Uncertain
Analysis performed using a standard panel of samples. No bands detected.
Figure description
Lane 1: Marker [kDa] 230, 130, 95, 72, 56, 36, 28, 17, 11 Lane 2: RT4 Lane 3: U-251 MG Lane 4: Human Plasma Lane 5: Liver Lane 6: Tonsil
Lane 1: Marker [kDa] 250, 130, 95, 72, 55, 36, 28, 17, 10 Lane 2: RT4 Lane 3: U-251 MG Lane 4: Human Plasma Lane 5: Liver Lane 6: Tonsil
Target mass (kDa)
215.3
215.3, 126
Antibody dilution
1:250
1:140
PROTEIN ARRAY
Antibody HPA017719
Antibody HPA056837
Standard validation
Supported
Pass with single peak corresponding to interaction only with its own antigen.
Supported
Pass with single peak corresponding to interaction only with its own antigen.
Figure description
Antibody specificity analysis with protein arrays. Predicted and matching interactions are shown in green.
Antibody specificity analysis with protein arrays. Predicted and matching interactions are shown in green.
