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Immunofluorescent staining of human cell line RH-30 shows localization to nucleoplasm & nuclear membrane.
Antibody dilution
1:35
Literature conformity
The subcellular location is partly supported by literature or no literature is available.
IMMUNOHISTOCHEMISTRY
Antibody HPA023911
Antibody HPA023950
Antibody HPA024815
Standard validation
Uncertain
Uncertain
Uncertain
Figure description
Immunohistochemical staining of human rectum shows strong nuclear and cytoplasmic positivity in glandular cells.
Immunohistochemical staining of human small intestine shows strong nuclear membrane positivity and slightly weaker cytoplasmic staining in glandular cells.
Immunohistochemical staining of human kidney shows strong cytoplasmic positivity in tubular cells.
Expression
RNA: detected in 3 tissues Protein: detected in 79 cell types
RNA: detected in 3 tissues Protein: detected in 72 cell types
RNA: detected in 3 tissues Protein: detected in 32 cell types
Retrieval
HIER pH6
HIER pH6
HIER pH6
Antibody dilution
1:150
1:150
1:150
Literature conformity
No avaliable gene/protein characterization data.
No avaliable gene/protein characterization data.
No avaliable gene/protein characterization data.
RNA consistency
Not consistent with RNA expression data.
Not consistent with RNA expression data.
Not consistent with RNA expression data.
WESTERN BLOT
Antibody HPA023911
Antibody HPA023950
Antibody HPA024815
Standard validation
Uncertain
Analysis performed using a standard panel of samples. Current setup is not applicable due to low RNA count.
Uncertain
Analysis performed using a standard panel of samples. Current setup is not applicable due to low RNA count.
Uncertain
Analysis performed using a standard panel of samples. Current setup is not applicable due to low RNA count.
Figure description
Target mass (kDa)
155.8
155.8
155.8
Antibody dilution
1:250
1:250
1:250
PROTEIN ARRAY
Antibody HPA023911
Antibody HPA023950
Antibody HPA024815
Standard validation
Supported
Pass with single peak corresponding to interaction only with its own antigen.
Supported
Pass with single peak corresponding to interaction only with its own antigen.
Supported
Pass with single peak corresponding to interaction only with its own antigen.
Figure description
Antibody specificity analysis with protein arrays. Predicted and matching interactions are shown in green.
Antibody specificity analysis with protein arrays. Predicted and matching interactions are shown in green.
Antibody specificity analysis with protein arrays. Predicted and matching interactions are shown in green.