NAPRT - Antibodies - The Human Protein Atlas

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NAPRT

TISSUE

CELL

CANCER

ANTIBODY INFORMATION

Antibody HPA023739

Antibody HPA024017

Antibody CAB068206

Antibody CAB068207

Provider

Atlas Antibodies
Sigma-Aldrich

Product name

HPA023739

HPA024017

AMAb90725

AMAb90823

Host species

Rabbit

Mouse

Clonality

pAb

mAb

Purity

Affinity purified using the PrEST-antigen as affinity ligand

Protein A/G

Other gene match

Released in version

References

VALIDATION SUMMARY

Antibody HPA023739

Antibody HPA024017

Antibody CAB068206

Antibody CAB068207

Immunocytochemistry

Immunohistochemistry

Western blot

Protein array

IMMUNOCYTOCHEMISTRY

Antibody HPA023739

Antibody HPA024017

Antibody CAB068206

Antibody CAB068207

Standard validation

Approved

Figure description

Immunofluorescent staining of human cell line U-251 MG shows localization to nucleoplasm, cytosol & the Golgi apparatus.

Antibody dilution

1:20

Literature conformity

The subcellular location is partly supported by literature or no literature is available.

IMMUNOHISTOCHEMISTRY

Antibody HPA023739

Antibody HPA024017

Antibody CAB068206

Antibody CAB068207

Formal validation: Independent

Validated

Spearman correlation >0.6 for protein expression in tissues using independent antibodies.

Validated

Spearman correlation >0.6 for protein expression in tissues using independent antibodies.

Figure description

Distribution of protein expression (antibody staining).
Spearman correlation with HPA024017 across 72 cell types.

Distribution of protein expression (antibody staining).
Spearman correlation with HPA023739 across 72 cell types.

Standard validation

Approved

Figure description

Immunohistochemical staining of human kidney shows nuclear and cytoplasmic positivity in tubular cells.

Immunohistochemical staining of human kidney shows strong cytoplasmic and nuclear positivity in cells in tubules.

Immunohistochemical staining of human rectum shows moderate cytoplasmic and nuclear positivity in glandular cells.

Expression

RNA: detected in 33 tissues
Protein: detected in 48 cell types

RNA: detected in 33 tissues
Protein: detected in 22 cell types

RNA: detected in 33 tissues
Protein: detected in 54 cell types

RNA: detected in 33 tissues
Protein: detected in 48 cell types

Retrieval

HIER pH6

Antibody dilution

1:75

1:25

1:2000

1:600

Literature conformity

Partly consistent with gene/protein characterization data.

Consistent with extensive gene/protein characterization data.

Partly consistent with gene/protein characterization data.

RNA consistency

Mainly consistent with RNA expression data.

Consistent with RNA expression data.

WESTERN BLOT

Antibody HPA023739

Antibody HPA024017

Antibody CAB068206

Antibody CAB068207

Standard validation

Supported

Analysis performed using a standard panel of samples.
Band of predicted size in kDa (+/-20%) with additional bands present.

Supported

Analysis performed using a standard panel of samples.
Single band corresponding to the predicted size in kDa (+/-20%).

Figure description

Lane 1: Marker [kDa] 230, 130, 95, 72, 56, 36, 28, 17, 11
Lane 2: RT4
Lane 3: U-251 MG
Lane 4: Human Plasma
Lane 5: Liver
Lane 6: Tonsil

Lane 1: Marker [kDa] 250, 130, 100, 70, 55, 35, 25, 15, 10
Lane 2: RT4
Lane 3: U-251 MG
Lane 4: Human Plasma
Lane 5: Liver
Lane 6: Tonsil

Target mass (kDa)

57.6, 56.1, 52.2

Antibody dilution

1:250

1:1000

PROTEIN ARRAY

Antibody HPA023739

Antibody HPA024017

Antibody CAB068206

Antibody CAB068207

Standard validation

Supported

Pass with single peak corresponding to interaction only with its own antigen.

Figure description

Antibody specificity analysis with protein arrays. Predicted and matching interactions are shown in green.

Antibody dilution

1:3000

1:500

ANTIGEN INFORMATION

Antibody HPA023739

Antibody HPA024017

Antibody CAB068206

Antibody CAB068207

Antigen

Recombinant protein fragment

Recombinant protein

Length (aa)

Antigen sequence

LLDTYSVWRSGLPNFLAVALALGELGYRAVGVRLDSGDLLQQAQEIRKVF
RAAAAQFQVPWLESVLIVVSNNIDEEALARLAQEGSEVNVIGIGTSV

PAFFEHLRALDCSEVTVRALPEGSLAFPGVPLLQVSGPLLVVQLLETPLL
CLVSYASLVATNAARLRLIAGP

Matching transcripts

NAPRT-002 - ENSP00000401508 [100%]
NAPRT-003 - ENSP00000390949 [100%]
NAPRT-004 - ENSP00000405670 [100%]

Other gene match

ANTIGEN VIEW

NAPRT-002	NAPRT-003	NAPRT-004

RELEVANT PUBLICATIONS

Antibody HPA023739

Antibody HPA024017

Antibody CAB068206

Antibody CAB068207

Mapping the subcellular protein distribution in three human cell lines
Fagerberg L et al
J Proteome Res 2011;10(8):3766-77

Dependence of tumor cell lines and patient-derived tumors on the NAD salvage pathway renders them sensitive to NAMPT inhibition with GNE-618
Xiao Y et al
Neoplasia 2013;15(10):1151-60

Extensive regulation of nicotinate phosphoribosyltransferase (NAPRT) expression in human tissues and tumors
Duarte-Pereira S et al
Oncotarget 2016;7(2):1973-83

Contact

contact@proteinatlas.org

The Project

The Human Protein Atlas

The Human Protein Atlas project is funded
by the Knut & Alice Wallenberg foundation.