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Immunofluorescent staining of human cell line Hep G2 shows localization to lipid droplets.
Antibody dilution
1:74
Literature conformity
The subcellular location is supported by literature.
IMMUNOHISTOCHEMISTRY
Antibody HPA016607
Antibody CAB037331
Standard validation
Supported
Supported
Figure description
Immunohistochemical staining of human lactating breast shows strong membranous positivity in glandular cells. Moderate cytoplasmic positivity was observed in ductal cells.
Immunohistochemical staining of human lactating breast shows strong membranous positivity in glandular cells.
Expression
RNA: detected in 37 tissues Protein: detected in 37 cell types
RNA: detected in 37 tissues Protein: detected in 20 cell types
Retrieval
HIER pH6
HIER pH6
Antibody dilution
1:50
1:30
Literature conformity
Consistent with extensive gene/protein characterization data.
Consistent with extensive gene/protein characterization data.
RNA consistency
Mainly consistent with RNA expression data.
Mainly consistent with RNA expression data.
WESTERN BLOT
Antibody HPA016607
Antibody CAB037331
Standard validation
Uncertain
Analysis performed using a standard panel of samples. Only bands not corresponding to the predicted size.
Supported
Analysis performed using a standard panel of samples. Single band corresponding to the predicted size in kDa (+/-20%).
Figure description
Lane 1: Marker [kDa] 250, 130, 100, 70, 55, 35, 25, 15, 10 Lane 2: RT4 Lane 3: U-251 MG Lane 4: Human Plasma Lane 5: Liver Lane 6: Tonsil
Target mass (kDa)
48.1
48.1, 13.7, 13.2, 11.9
Antibody dilution
1:250
1:500
PROTEIN ARRAY
Antibody HPA016607
Antibody CAB037331
Standard validation
Approved
Pass with quality comment low specificity (binding to 1-2 PrESTs >15% and <40%).
Figure description
Antibody specificity analysis with protein arrays. Predicted and matching interactions are shown in green.
