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CCDC81

ANTIBODY INFORMATION
 
 

Antibody HPA040745

  

Antibody HPA040827

  

Provider

 Atlas Antibodies
Sigma-Aldrich
   Atlas Antibodies
Sigma-Aldrich
  

Product name

 HPA040745   HPA040827  

Host species

 Rabbit   Rabbit  

Clonality

 pAb   pAb  

Purity

 Affinity purified using the PrEST-antigen as affinity ligand   Affinity purified using the PrEST-antigen as affinity ligand  

Other gene match

    

Released in version

 9   9  

References

    

VALIDATION SUMMARY
 
 

Antibody HPA040745

  

Antibody HPA040827

  
Immunocytochemistry

Uncertain    
Immunohistochemistry

Uncertain   Uncertain  
Western blot

Uncertain   Uncertain  
Protein array

Supported   Supported  

IMMUNOCYTOCHEMISTRY
 
 

Antibody HPA040745

  

Antibody HPA040827

  

Standard validation

 Uncertain    

   

Figure description

 Immunofluorescent staining of human cell line A-431 shows localization to plasma membrane.    

Antibody dilution

 1:112    

Literature conformity

 The subcellular location is not consistent with literature.    

IMMUNOHISTOCHEMISTRY
 
 

Antibody HPA040745

  

Antibody HPA040827

  

Standard validation

 Uncertain   Uncertain  

   

Figure description

 Immunohistochemical staining of human kidney shows strong cytoplasmic and membranous positivity in cells of tubules.   Immunohistochemical staining of human kidney shows moderate membranous and cytoplasmic positivity in tubules.  

Expression

 RNA: detected in 6 tissues
Protein: detected in 69 cell types		   RNA: detected in 6 tissues
Protein: detected in 61 cell types		  

Retrieval

 HIER pH6   HIER pH6  

Antibody dilution

 1:400   1:250  

Literature conformity

 No avaliable gene/protein characterization data.   No avaliable gene/protein characterization data.  

RNA consistency

 Not consistent with RNA expression data.   Not consistent with RNA expression data.  

WESTERN BLOT
 
 

Antibody HPA040745

  

Antibody HPA040827

  

Standard validation

 Uncertain

Analysis performed using a standard panel of samples.
Current setup is not applicable due to low RNA count.		   Uncertain

Analysis performed using a standard panel of samples.
Current setup is not applicable due to low RNA count.		  

   

Figure description

    

Target mass (kDa)

 76.1, 66.1, 45.5   76.1, 66.1, 45.5  

Antibody dilution

 1:250   1:250  

PROTEIN ARRAY
 
 

Antibody HPA040745

  

Antibody HPA040827

  

Standard validation

 Supported

Pass with single peak corresponding to interaction only with its own antigen.		   Supported

Pass with single peak corresponding to interaction only with its own antigen.		  

   

Figure description

 Antibody specificity analysis with protein arrays. Predicted and matching interactions are shown in green.   Antibody specificity analysis with protein arrays. Predicted and matching interactions are shown in green.  

Antibody dilution

 1:3000   1:3000  

ANTIGEN INFORMATION
 
 

Antibody HPA040745

  

Antibody HPA040827

  

Antigen

 Recombinant protein fragment   Recombinant protein fragment  

Length (aa)

 102   86  

Antigen sequence

 
NHKNEKPEFYKSFLFDKRPLSPALNALKQEEYSRSLLKQMDNRQENEIKQ
RQYRELMDRLEQVQLTEELAAQRAKFLKDKMEETQCYKRALDAQIKNKPS
RL
  
SEPIFGKNEGELMVEKQKREQNYMKHQLEAAANHKRKAILHQLVDQRRDL
QMLQRTQREHLADRTAELERVNRVNQCLQEDWERSA
 

Matching transcripts

 CCDC81-001 - ENSP00000346800 [100%]
CCDC81-002 - ENSP00000415528 [100%]
CCDC81-003 - ENSP00000437165 [100%]
  CCDC81-001 - ENSP00000346800 [100%]
CCDC81-002 - ENSP00000415528 [100%]
CCDC81-003 - ENSP00000437165 [100%]
 

Other gene match

    
ANTIGEN VIEW
CCDC81-001
CCDC81-002
CCDC81-003
 

Contact

The Project

The Human Protein Atlas

The Human Protein Atlas project is funded
by the Knut & Alice Wallenberg foundation.