ENDOD1 - Antibodies - The Human Protein Atlas

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ENDOD1

TISSUE

CELL

CANCER

ANTIBODY INFORMATION

Antibody HPA008932

Antibody HPA010517

Provider

Atlas Antibodies
Sigma-Aldrich

Product name

HPA008932

HPA010517

Host species

Rabbit

Clonality

pAb

Purity

Affinity purified using the PrEST-antigen as affinity ligand

Other gene match

Released in version

References

VALIDATION SUMMARY

Antibody HPA008932

Antibody HPA010517

Immunocytochemistry

Approved

Immunohistochemistry

Western blot

Supported

Uncertain

Protein array

Supported

IMMUNOCYTOCHEMISTRY

Antibody HPA008932

Antibody HPA010517

Standard validation

Approved

Figure description

Immunofluorescent staining of human cell line RT4 shows localization to nucleoplasm & nuclear membrane.

Antibody dilution

1:50

Literature conformity

The subcellular location is partly supported by literature or no literature is available.

IMMUNOHISTOCHEMISTRY

Antibody HPA008932

Antibody HPA010517

Formal validation: Orthogonal

Validated

Pearson correlation >0.6 for protein and RNA expression in cell lines.

Validated

Pearson correlation >0.6 for protein and RNA expression in cell lines.

Figure description

Distribution of protein expression (antibody staining) and RNA expression (TPM) across 44 cell lines.

Formal validation: Independent

Validated

Pearson correlation >0.6 for protein expression in cell lines using independent antibodies.

Validated

Pearson correlation >0.6 for protein expression in cell lines using independent antibodies.

Figure description

Distribution of protein expression (antibody staining).
Pearson correlation with HPA010517 across 46 cell lines.

Distribution of protein expression (antibody staining).
Pearson correlation with HPA008932 across 46 cell lines.

Formal validation: Independent

Validated

Spearman correlation >0.6 for protein expression in tissues using independent antibodies.

Validated

Spearman correlation >0.6 for protein expression in tissues using independent antibodies.

Figure description

Distribution of protein expression (antibody staining).
Spearman correlation with HPA010517 across 74 cell types.

Distribution of protein expression (antibody staining).
Spearman correlation with HPA008932 across 74 cell types.

Standard validation

Supported

Figure description

Immunohistochemical staining of human prostate shows strong cytoplasmic positivity in glandular cells.

Expression

RNA: detected in 37 tissues
Protein: detected in 69 cell types

RNA: detected in 37 tissues
Protein: detected in 66 cell types

Retrieval

HIER pH6

Antibody dilution

1:1100

1:650

Literature conformity

No avaliable gene/protein characterization data.

RNA consistency

Mainly consistent with RNA expression data.

WESTERN BLOT

Antibody HPA008932

Antibody HPA010517

Standard validation

Supported

Analysis performed using a standard panel of samples.
Band of predicted size in kDa (+/-20%) with additional bands present.

Uncertain

Analysis performed using a standard panel of samples.
Weak band of predicted size but with additional bands of higher intensity also present.

Figure description

Lane 1: Marker [kDa] 230, 130, 95, 72, 56, 36, 28, 17, 11
Lane 2: RT4
Lane 3: U-251 MG
Lane 4: Human Plasma
Lane 5: Liver
Lane 6: Tonsil

Target mass (kDa)

Antibody dilution

1:250

PROTEIN ARRAY

Antibody HPA008932

Antibody HPA010517

Standard validation

Supported

Pass with single peak corresponding to interaction only with its own antigen.

Figure description

Antibody specificity analysis with protein arrays. Predicted and matching interactions are shown in green.

Antibody dilution

1:3000

ANTIGEN INFORMATION

Antibody HPA008932

Antibody HPA010517

Antigen

Recombinant protein fragment

Length (aa)

148

139

Antigen sequence

SGEDLYILTGTVPSDYRVKDKVAVPEFVWLAACCAVPGGGWAMGFVKHTR
DSDIIEDVMVKDLQKLLPFNPQLFQNNCGETEQDTEKMKKILEVVNQIQD
EERMVQSQKSSSPLSSTRSKRSTLLPPEASEGSSSFLGKLMGFIATPF

GECDKFFYAGTPPAGLAADSHVKICQRAEGAERFATLYSTRDRIPVYSAF
RAPRPAPGGAEQRWLVEPQIDDPNSNLEEAINEAEAITSVNSLGSKQALN
TDYLDSDYQRGQLYPFSLSSDVQVATFTLTNSAPMTQSF

Matching transcripts

ENDOD1-001 - ENSP00000278505 [100%]

Other gene match

ANTIGEN VIEW

ENDOD1-001

Contact

contact@proteinatlas.org

The Project

The Human Protein Atlas

The Human Protein Atlas project is funded
by the Knut & Alice Wallenberg foundation.