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Immunofluorescent staining of human cell line U-2 OS shows localization to microtubules.
Antibody dilution
1:103
Literature conformity
The subcellular location is partly supported by literature or no literature is available.
IMMUNOHISTOCHEMISTRY
Antibody HPA051043
Antibody HPA056293
Standard validation
Uncertain
Figure description
Immunohistochemical staining of human bronchus shows moderate cytoplasmic positivity in respiratory epithelial cells.
Expression
RNA: detected in 37 tissues Protein: detected in 58 cell types
Retrieval
HIER pH6
Antibody dilution
1:1500
Literature conformity
Partly consistent with gene/protein characterization data.
RNA consistency
Mainly not consistent with RNA expression data.
WESTERN BLOT
Antibody HPA051043
Antibody HPA056293
Standard validation
Uncertain
Analysis performed using a standard panel of samples. Only bands not corresponding to the predicted size.
Uncertain
Analysis performed using a standard panel of samples. Single band larger than predicted size in kDa (+20%) but partly supported by experimental and/or bioinformatic data.
Figure description
Lane 1: Marker [kDa] 250, 130, 95, 72, 55, 36, 28, 17, 10 Lane 2: RT4 Lane 3: U-251 MG Lane 4: Human Plasma Lane 5: Liver Lane 6: Tonsil
Target mass (kDa)
41.2
41.2
Antibody dilution
1:520
1:410
PROTEIN ARRAY
Antibody HPA051043
Antibody HPA056293
Standard validation
Approved
Pass with quality comment low specificity (binding to 1-2 PrESTs >15% and <40%).
Supported
Pass with single peak corresponding to interaction only with its own antigen.
Figure description
Antibody specificity analysis with protein arrays. Predicted and matching interactions are shown in green.
Antibody specificity analysis with protein arrays. Predicted and matching interactions are shown in green.
