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Immunofluorescent staining of human cell line HaCaT shows localization to plasma membrane.
Antibody dilution
1:24
Literature conformity
The subcellular location is supported by literature.
IMMUNOHISTOCHEMISTRY
Antibody HPA014908
Antibody HPA058444
Standard validation
Approved
Figure description
Immunohistochemical staining of human colon shows strong cytoplasmic and membranous positivity in glandular cells.
Expression
RNA: detected in 3 tissues Protein: detected in 31 cell types
Retrieval
HIER pH6
Antibody dilution
1:400
Literature conformity
Partly consistent with extensive gene/protein characterization data.
RNA consistency
Mainly not consistent with RNA expression data.
WESTERN BLOT
Antibody HPA014908
Antibody HPA058444
Standard validation
Uncertain
Analysis performed using a standard panel of samples. Single band larger than predicted size in kDa (+20%) but partly supported by experimental and/or bioinformatic data.
Supported
Analysis performed using a standard panel of samples. Single band corresponding to the predicted size in kDa (+/-20%).
Figure description
Lane 1: Marker [kDa] 230, 130, 95, 72, 56, 36, 28, 17, 11 Lane 2: RT4 Lane 3: U-251 MG Lane 4: Human Plasma Lane 5: Liver Lane 6: Tonsil
Lane 1: Marker [kDa] 250, 130, 95, 72, 55, 36, 28, 17, 10 Lane 2: RT4 Lane 3: U-251 MG Lane 4: Human Plasma Lane 5: Liver Lane 6: Tonsil
Target mass (kDa)
88.3, 82.8, 82.7, 58.9, 46.7
88.3, 82.8, 82.7
Antibody dilution
1:250
1:80
PROTEIN ARRAY
Antibody HPA014908
Antibody HPA058444
Standard validation
Supported
Pass with single peak corresponding to interaction only with its own antigen.
Supported
Pass with single peak corresponding to interaction only with its own antigen.
Figure description
Antibody specificity analysis with protein arrays. Predicted and matching interactions are shown in green.
Antibody specificity analysis with protein arrays. Predicted and matching interactions are shown in green.
