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CCDC83

ANTIBODY INFORMATION
 
 

Antibody HPA039707

  

Antibody HPA039882

  

Provider

 Atlas Antibodies
Sigma-Aldrich
   Atlas Antibodies
Sigma-Aldrich
  

Product name

 HPA039707   HPA039882  

Host species

 Rabbit   Rabbit  

Clonality

 pAb   pAb  

Purity

 Affinity purified using the PrEST-antigen as affinity ligand   Affinity purified using the PrEST-antigen as affinity ligand  

Other gene match

    

Released in version

 8   8  

References

    

VALIDATION SUMMARY
 
 

Antibody HPA039707

  

Antibody HPA039882

  
Immunocytochemistry

Approved   Approved  
Western blot

Uncertain   Uncertain  
Protein array

Approved   Supported  

IMMUNOCYTOCHEMISTRY
 
 

Antibody HPA039707

  

Antibody HPA039882

  

Standard validation

 Approved   Approved  

   

Figure description

 Immunofluorescent staining of human cell line A-431 shows localization to plasma membrane & cytosol.   Immunofluorescent staining of human cell line A-431 shows localization to nucleoplasm & cytosol.  

Antibody dilution

 1:130   1:192  

Literature conformity

 The subcellular location is partly supported by literature or no literature is available.   The subcellular location is partly supported by literature or no literature is available.  

WESTERN BLOT
 
 

Antibody HPA039707

  

Antibody HPA039882

  

Standard validation

 Uncertain

Analysis performed using a standard panel of samples.
Current setup is not applicable due to low RNA count.		   Uncertain

Analysis performed using a standard panel of samples.
Current setup is not applicable due to low RNA count.		  

   

Figure description

    

Target mass (kDa)

 52.4, 48.9   52.4, 48.9, 37.8  

Antibody dilution

 1:250   1:250  

PROTEIN ARRAY
 
 

Antibody HPA039707

  

Antibody HPA039882

  

Standard validation

 Approved

Pass with quality comment low specificity (binding to 1-2 PrESTs >15% and <40%).		   Supported

Pass with single peak corresponding to interaction only with its own antigen.		  

   

Figure description

 Antibody specificity analysis with protein arrays. Predicted and matching interactions are shown in green.   Antibody specificity analysis with protein arrays. Predicted and matching interactions are shown in green.  

Antibody dilution

 1:6000   1:6000  

ANTIGEN INFORMATION
 
 

Antibody HPA039707

  

Antibody HPA039882

  

Antigen

 Recombinant protein fragment   Recombinant protein fragment  

Length (aa)

 101   98  

Antigen sequence

 
MENSGKANKKDTHDGPPKEIKLPTSEALLDYQCQIKEDAVEQFMFQIKTL
RKKNQKYHERNSRLKEEQIWHIRHLLKELSEEKAEGLPVVTREDVEEAMK
E
  
STILHLSHENSIEDLQYVKIDKEENSGTEFGDTDMKYLLYEDEKDFKDYV
NLGPLGVKLMSVESKKMPIHFQEKEIPVKLYKDVRSPESHITYKMMKS
 

Matching transcripts

 CCDC83-001 - ENSP00000280245 [100%]
CCDC83-002 - ENSP00000344512 [100%]
  CCDC83-001 - ENSP00000280245 [100%]
CCDC83-002 - ENSP00000344512 [100%]
CCDC83-003 - ENSP00000434373 [100%]
 

Other gene match

    
ANTIGEN VIEW
CCDC83-001
CCDC83-002
CCDC83-003
 

Contact

The Project

The Human Protein Atlas

The Human Protein Atlas project is funded
by the Knut & Alice Wallenberg foundation.