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CCT5

ANTIBODY INFORMATION
 
 

Antibody HPA002238

  

Antibody HPA005958

  

Antibody CAB006271

  

Provider

 Atlas Antibodies
Sigma-Aldrich
   Atlas Antibodies
Sigma-Aldrich
   Abnova
  

Product name

 HPA002238   HPA005958   H00022948-M01  

Host species

 Rabbit   Rabbit   Mouse  

Clonality

 pAb   pAb   mAb  

Purity

 Affinity purified using the PrEST-antigen as affinity ligand   Affinity purified using the PrEST-antigen as affinity ligand   Protein A/G  

Other gene match

      

Released in version

 3   4   3  

References

      

VALIDATION SUMMARY
 
 

Antibody HPA002238

  

Antibody HPA005958

  

Antibody CAB006271

  
Immunocytochemistry

  Supported    
Immunohistochemistry

Supported   Supported   Supported  
Western blot

Supported   Supported   Supported  
Protein array

Supported   Supported    

IMMUNOCYTOCHEMISTRY
 
 

Antibody HPA002238

  

Antibody HPA005958

  

Antibody CAB006271

  

Standard validation

   Supported    

     

Figure description

   Immunofluorescent staining of human cell line U-2 OS shows localization to nucleoli & cytosol.    

Antibody dilution

   1:11    

Literature conformity

   The subcellular location is supported by literature.    

IMMUNOHISTOCHEMISTRY
 
 

Antibody HPA002238

  

Antibody HPA005958

  

Antibody CAB006271

  

Standard validation

 Supported   Supported   Supported  

     

Figure description

 Immunohistochemical staining of human testis shows strong cytoplasmic positivity in cells in seminiferus ducts.   Immunohistochemical staining of human testis shows strong cytoplasmic positivity in cells in seminiferus ducts.   Immunohistochemical staining of human testis shows strong cytoplasmic positivity in cells in seminiferus ducts.  

Expression

 RNA: detected in 37 tissues
Protein: detected in 58 cell types		   RNA: detected in 37 tissues
Protein: detected in 50 cell types		   RNA: detected in 37 tissues
Protein: detected in 65 cell types		  

Retrieval

 HIER pH6   HIER pH6   HIER pH6  

Antibody dilution

 1:25   1:30   1:10000  

Literature conformity

 Consistent with extensive gene/protein characterization data.   Consistent with extensive gene/protein characterization data.   Consistent with extensive gene/protein characterization data.  

RNA consistency

 Consistent with RNA expression data.   Consistent with RNA expression data.   Consistent with RNA expression data.  

WESTERN BLOT
 
 

Antibody HPA002238

  

Antibody HPA005958

  

Antibody CAB006271

  

Standard validation

 Supported

Analysis performed using a standard panel of samples.
Single band corresponding to the predicted size in kDa (+/-20%).		   Supported

Analysis performed using a standard panel of samples.
Band of predicted size in kDa (+/-20%) with additional bands present.		   Supported

Analysis performed using a standard panel of samples.
Single band corresponding to the predicted size in kDa (+/-20%).		  

     

Figure description

 Lane 1: Marker [kDa] 220, 112, 84, 47, 32, 26, 16.8
Lane 2: RT4
Lane 3: U-251 MG
Lane 4: Human Plasma
Lane 5: Liver
Lane 6: Tonsil		   Lane 1: Marker [kDa] 220, 112, 84, 47, 32, 26, 16.8
Lane 2: RT4
Lane 3: U-251 MG
Lane 4: Human Plasma
Lane 5: Liver
Lane 6: Tonsil		   Lane 1: Marker [kDa] 220, 112, 84, 47, 32, 26, 16.8
Lane 2: RT4
Lane 3: U-251 MG
Lane 4: Human Plasma
Lane 5: Liver
Lane 6: Tonsil		  

Target mass (kDa)

 59.7, 57.1, 55.3, 53.8, 49.5   59.7, 57.1, 55.3, 53.8, 49.5   59.7, 57.1, 55.3, 53.8, 49.5, 4.9  

Antibody dilution

 1:250   1:250   1:500  

PROTEIN ARRAY
 
 

Antibody HPA002238

  

Antibody HPA005958

  

Antibody CAB006271

  

Standard validation

 Supported

Pass with single peak corresponding to interaction only with its own antigen.		   Supported

Pass with single peak corresponding to interaction only with its own antigen.		    

     

Figure description

 Antibody specificity analysis with protein arrays. Predicted and matching interactions are shown in green.   Antibody specificity analysis with protein arrays. Predicted and matching interactions are shown in green.    

Antibody dilution

 1:500   1:500    

ANTIGEN INFORMATION
 
 

Antibody HPA002238

  

Antibody HPA005958

  

Antibody CAB006271

  

Antigen

 Recombinant protein fragment   Recombinant protein fragment   Recombinant protein  

Length (aa)

 116   149    

Antigen sequence

 
LDRGIHPIRIADGYEQAARVAIEHLDKISDSVLVDIKDTEPLIQTAKTTL
GSKVVNSCHRQMAEIAVNAVLTVADMERRDVDFELIKVEGKVGGRLEDTK
LIKGVIVDKDFSHPQM
  
ALQKYEKEKFEEMIQQIKETGANLAICQWGFDDEANHLLLQNNLPAVRWV
GGPEIELIAIATGGRIVPRFSELTAEKLGFAGLVQEISFGTTKDKMLVIE
QCKNSRAVTIFIRGGNKMIIEEAKRSLHDALCVIRNLIRDNRVVYGGGA
  

											 
																				

																		

										
											
Matching transcripts

												
																					CCT5-001 - ENSP00000280326 [100%] 
CCT5-002 - ENSP00000423318 [100%] 
CCT5-003 - ENSP00000426923 [100%] 
CCT5-013 - ENSP00000427297 [100%] 
CCT5-014 - ENSP00000423052 [100%] 

											 
																						CCT5-001 - ENSP00000280326 [100%] 
CCT5-002 - ENSP00000423318 [100%] 
CCT5-003 - ENSP00000426923 [100%] 
CCT5-013 - ENSP00000427297 [100%] 
CCT5-014 - ENSP00000423052 [100%] 

											 
																						
											 
																				

																		

										
											
Other gene match

												
																					
											 
																						
											 
																						
											 
																				

																		

										ANTIGEN VIEW
									
									
										

		
			

				

										
						

							

								

									
CCT5-001
CCT5-002
CCT5-003
CCT5-013
CCT5-014
								

							

						

							
									

			

				
	

	

		
					
	

													
															
				
						

				 
			

		

			
	











	

		


			

				

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