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INPP1

ANTIBODY INFORMATION
 
 

Antibody HPA036698

  

Antibody HPA036699

  

Provider

 Atlas Antibodies
Sigma-Aldrich
   Atlas Antibodies
Sigma-Aldrich
  

Product name

 HPA036698   HPA036699  

Host species

 Rabbit   Rabbit  

Clonality

 pAb   pAb  

Purity

 Affinity purified using the PrEST-antigen as affinity ligand   Affinity purified using the PrEST-antigen as affinity ligand  

Other gene match

    

Released in version

 7   12  

References

    

VALIDATION SUMMARY
 
 

Antibody HPA036698

  

Antibody HPA036699

  
Immunocytochemistry

Approved   Approved  
Immunohistochemistry

Supported   Supported  
Western blot

Uncertain   Uncertain  
Protein array

Approved   Supported  

IMMUNOCYTOCHEMISTRY
 
 

Antibody HPA036698

  

Antibody HPA036699

  

Standard validation

 Approved   Approved  

   

Figure description

 Immunofluorescent staining of human cell line U-251 MG shows localization to microtubule organizing center.   Immunofluorescent staining of human cell line U-2 OS shows localization to vesicles.  

Antibody dilution

 1:76   1:85  

Literature conformity

 The subcellular location is partly supported by literature or no literature is available.   The subcellular location is partly supported by literature or no literature is available.  

IMMUNOHISTOCHEMISTRY
 
 

Antibody HPA036698

  

Antibody HPA036699

  

Standard validation

 Supported   Supported  

   

Figure description

 Immunohistochemical staining of human esophagus shows cytoplasmic and a few cases of nuclear positivity in squamous epithelial cells.   Immunohistochemical staining of human kidney shows strong cytoplasmic positivity in tubular cells.  

Expression

 RNA: detected in 37 tissues
Protein: detected in 29 cell types		   RNA: detected in 37 tissues
Protein: detected in 70 cell types		  

Retrieval

 HIER pH6   HIER pH6  

Antibody dilution

 1:750   1:100  

Literature conformity

 Partly consistent with gene/protein characterization data.   Partly consistent with gene/protein characterization data.  

RNA consistency

 Mainly consistent with RNA expression data.   Mainly consistent with RNA expression data.  

WESTERN BLOT
 
 

Antibody HPA036698

  

Antibody HPA036699

  

Standard validation

 Uncertain

Analysis performed using a standard panel of samples.
Weak band of predicted size but with additional bands of higher intensity also present.		   Uncertain

Analysis performed using a standard panel of samples.
No bands detected.		  

   

Figure description

   Lane 1: Marker [kDa] 230, 130, 95, 72, 56, 36, 28, 17, 11
Lane 2: RT4
Lane 3: U-251 MG
Lane 4: Human Plasma
Lane 5: Liver
Lane 6: Tonsil		  

Target mass (kDa)

 44, 23.8, 23.6, 21.3, 17.5, 16.1, 10.2   44  

Antibody dilution

 1:250   1:250  

PROTEIN ARRAY
 
 

Antibody HPA036698

  

Antibody HPA036699

  

Standard validation

 Approved

Pass with quality comment low specificity (binding to 1-2 PrESTs >15% and <40%).		   Supported

Pass with single peak corresponding to interaction only with its own antigen.		  

   

Figure description

 Antibody specificity analysis with protein arrays. Predicted and matching interactions are shown in green.   Antibody specificity analysis with protein arrays. Predicted and matching interactions are shown in green.  

Antibody dilution

 1:3000   1:6000  

ANTIGEN INFORMATION
 
 

Antibody HPA036698

  

Antibody HPA036699

  

Antigen

 Recombinant protein fragment   Recombinant protein fragment  

Length (aa)

 81   88  

Antigen sequence

 
RELLCVSEKAANIARACRQQEALFQLLIEEKKEGEKNKKFAVDFKTLADV
LVQEVIKQNMENKFPGLEKNIFGEESNEFTN
  
TSEKETIKAALSRVCGDRIFGAAGAGYKSLCVVQGLVDIYIFSEDTTFKW
DSCAAHAILRAMGGGIVDLKECLERNPETGLDLPQLVY
 

Matching transcripts

 INPP1-001 - ENSP00000325423 [100%]
INPP1-002 - ENSP00000404732 [100%]
INPP1-003 - ENSP00000392814 [100%]
INPP1-006 - ENSP00000391415 [100%]
INPP1-007 - ENSP00000409786 [100%]
INPP1-009 - ENSP00000395424 [100%]
INPP1-014 - ENSP00000387079 [100%]
INPP1-201 - ENSP00000376142 [100%]
  INPP1-001 - ENSP00000325423 [100%]
INPP1-201 - ENSP00000376142 [100%]
 

Other gene match

    
ANTIGEN VIEW
INPP1-001
INPP1-002
INPP1-003
INPP1-006
INPP1-007
INPP1-009
INPP1-014
INPP1-201
 

Contact

The Project

The Human Protein Atlas

The Human Protein Atlas project is funded
by the Knut & Alice Wallenberg foundation.