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ATP5A1

ANTIBODY INFORMATION
 
 

Antibody HPA040622

  

Antibody HPA044202

  

Antibody CAB013067

  

Provider

 Atlas Antibodies
Sigma-Aldrich
   Atlas Antibodies
Sigma-Aldrich
   Molecular Probes
  

Product name

 HPA040622   HPA044202   A-21350  

Host species

 Rabbit   Rabbit   Mouse  

Clonality

 pAb   pAb   mAb  

Purity

 Affinity purified using the PrEST-antigen as affinity ligand   Affinity purified using the PrEST-antigen as affinity ligand   Not known  

Other gene match

      

Released in version

 13   13   4  

References

      

VALIDATION SUMMARY
 
 

Antibody HPA040622

  

Antibody HPA044202

  

Antibody CAB013067

  
Immunocytochemistry

    Supported  
Immunohistochemistry

Supported   Supported   Supported  
Western blot

Supported   Supported   Supported  
Protein array

Supported   Supported    

IMMUNOCYTOCHEMISTRY
 
 

Antibody HPA040622

  

Antibody HPA044202

  

Antibody CAB013067

  

Standard validation

     Supported  

     

Figure description

     Immunofluorescent staining of human cell line U-2 OS shows localization to mitochondria.  

Antibody dilution

     1:37  

Literature conformity

     The subcellular location is supported by literature.  

IMMUNOHISTOCHEMISTRY
 
 

Antibody HPA040622

  

Antibody HPA044202

  

Antibody CAB013067

  

Standard validation

 Supported   Supported   Supported  

     

Figure description

 Immunohistochemical staining of human heart muscle shows strong cytoplasmic positivity in myocytes.   Immunohistochemical staining of human heart muscle shows strong cytoplasmic positivity in myocytes.   Immunohistochemical staining of human heart muscle shows strong cytoplasmic positivity in myocytes.  

Expression

 RNA: detected in 37 tissues
Protein: detected in 80 cell types		   RNA: detected in 37 tissues
Protein: detected in 79 cell types		   RNA: detected in 37 tissues
Protein: detected in 81 cell types		  

Retrieval

 HIER pH6   HIER pH6   HIER pH6  

Antibody dilution

 1:40   1:100   1:150  

Literature conformity

 Consistent with extensive gene/protein characterization data.   Consistent with extensive gene/protein characterization data.   Partly consistent with extensive gene/protein characterization data.  

RNA consistency

 Consistent with RNA expression data.   Consistent with RNA expression data.   Consistent with RNA expression data.  

WESTERN BLOT
 
 

Antibody HPA040622

  

Antibody HPA044202

  

Antibody CAB013067

  

Standard validation

 Supported

Analysis performed using a standard panel of samples.
Single band corresponding to the predicted size in kDa (+/-20%).		   Supported

Analysis performed using a standard panel of samples.
Single band corresponding to the predicted size in kDa (+/-20%).		   Supported

Analysis performed using a standard panel of samples.
Band of predicted size in kDa (+/-20%) with additional bands present.		  

     

Figure description

 Lane 1: Marker [kDa] 250, 130, 95, 72, 55, 36, 28, 17, 10
Lane 2: RT4
Lane 3: U-251 MG
Lane 4: Human Plasma
Lane 5: Liver
Lane 6: Tonsil		   Lane 1: Marker [kDa] 250, 130, 95, 72, 55, 36, 28, 17, 10
Lane 2: RT4
Lane 3: U-251 MG
Lane 4: Human Plasma
Lane 5: Liver
Lane 6: Tonsil		   Lane 1: Marker [kDa] 213, 118, 83, 50, 33, 26, 18
Lane 2: RT4
Lane 3: U-251 MG
Lane 4: Human Plasma
Lane 5: Liver
Lane 6: Tonsil		  

Target mass (kDa)

 59.7, 57.5, 54.5, 21.3   59.7, 57.5, 54.5, 22.2, 21.6, 15.3, 11.7   59.7, 57.5, 54.5, 22.2, 21.6, 21.3, 15.3, 11.7  

Antibody dilution

 1:250   1:250   1:500  

PROTEIN ARRAY
 
 

Antibody HPA040622

  

Antibody HPA044202

  

Antibody CAB013067

  

Standard validation

 Supported

Pass with single peak corresponding to interaction only with its own antigen.		   Supported

Pass with single peak corresponding to interaction only with its own antigen.		    

     

Figure description

 Antibody specificity analysis with protein arrays. Predicted and matching interactions are shown in green.   Antibody specificity analysis with protein arrays. Predicted and matching interactions are shown in green.    

Antibody dilution

 1:500   1:500    

ANTIGEN INFORMATION
 
 

Antibody HPA040622

  

Antibody HPA044202

  

Antibody CAB013067

  

Antigen

 Recombinant protein fragment   Recombinant protein fragment   Not known  

Length (aa)

 85   100    

Antigen sequence

 
QRELIIGDRQTGKTSIAIDTIINQKRFNDGSDEKKKLYCIYVAIGQKRST
VAQLVKRLTDADAMKYTIVVSATASDAAPLQYLAP
  
SILEERILGADTSVDLEETGRVLSIGDGIARVHGLRNVQAEEMVEFSSGL
KGMSLNLEPDNVGVVVFGNDKLIKEGDIVKRTGAIVDVPVGEELLGRVVD
  

											 
																				

																		

										
											
Matching transcripts

												
																					ATP5A1-001 - ENSP00000381736 [100%] 
ATP5A1-009 - ENSP00000467037 [100%] 
ATP5A1-201 - ENSP00000282050 [100%] 
ATP5A1-202 - ENSP00000465477 [100%] 
ATP5A1-018 - ENSP00000466975 [98%] 

											 
																						ATP5A1-001 - ENSP00000381736 [100%] 
ATP5A1-003 - ENSP00000465497 [100%] 
ATP5A1-004 - ENSP00000465259 [100%] 
ATP5A1-005 - ENSP00000468458 [100%] 
ATP5A1-016 - ENSP00000467830 [100%] 
ATP5A1-201 - ENSP00000282050 [100%] 
ATP5A1-202 - ENSP00000465477 [100%] 
ATP5A1-009 - ENSP00000467037 [91%] 

											 
																						
											 
																				

																		

										
											
Other gene match

												
																					
											 
																						
											 
																						
											 
																				

																		

										ANTIGEN VIEW
									
									
										

		
			

				

										
						

							

								

									
ATP5A1-001
ATP5A1-003
ATP5A1-004
ATP5A1-005
ATP5A1-009
ATP5A1-016
ATP5A1-018
ATP5A1-201
ATP5A1-202
								

							

						

							
									

			

				
	

	

		
					
	

													
															
				
						

				 
			

		

			
	











	

		


			

				

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