PTH - Antibodies - The Human Protein Atlas

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PTH

TISSUE

CELL

CANCER

ANTIBODY INFORMATION

Antibody HPA048540

Antibody CAB000072

Antibody CAB023409

Antibody CAB026363

Provider

Atlas Antibodies
Sigma-Aldrich

Novocastra

AbD Serotec

SDIX

Product name

HPA048540

Novocastra-PTH-OP4

7170-6206

3466.00.02

Host species

Rabbit

Mouse

Rabbit

Clonality

pAb

mAb

msAb

Purity

Affinity purified using the PrEST-antigen as affinity ligand

Not known

Protein A/G

Affinity

Other gene match

Released in version

References

VALIDATION SUMMARY

Antibody HPA048540

Antibody CAB000072

Antibody CAB023409

Antibody CAB026363

Immunohistochemistry

Western blot

Protein array

IMMUNOHISTOCHEMISTRY

Antibody HPA048540

Antibody CAB000072

Antibody CAB023409

Antibody CAB026363

Standard validation

Supported

Figure description

Immunohistochemical staining of human parathyroid gland shows strong cytoplasmic and membranous positivity in glandular cells.

Immunohistochemical staining of human parathyroid gland shows strong cytoplasmic positivity in glandular cells.

Expression

RNA: detected in 2 tissues
Protein: detected in 4 cell types

RNA: detected in 2 tissues
Protein: detected in 2 cell types

RNA: detected in 2 tissues
Protein: detected in 3 cell types

RNA: detected in 2 tissues
Protein: detected in 2 cell types

Retrieval

HIER pH6

HIER pH9

HIER pH6

Antibody dilution

1:2000

1:1000

1:5000

1:1200

Literature conformity

Consistent with extensive gene/protein characterization data.

RNA consistency

Consistent with RNA expression data.

WESTERN BLOT

Antibody HPA048540

Antibody CAB000072

Antibody CAB023409

Antibody CAB026363

Standard validation

Uncertain

Analysis performed using a standard panel of samples.
Only bands not corresponding to the predicted size.

Supported

Analysis performed using a standard panel of samples.
Band of predicted size in kDa (+/-20%) with additional bands present.

Uncertain

Analysis performed using a standard panel of samples.
No bands detected.

Uncertain

Analysis performed using a standard panel of samples.
Only bands not corresponding to the predicted size.

Figure description

Lane 1: Marker [kDa] 250, 130, 95, 72, 55, 36, 28, 17, 11
Lane 2: RT4
Lane 3: U-251 MG
Lane 4: Human Plasma
Lane 5: Liver
Lane 6: Tonsil

Target mass (kDa)

12.9

Antibody dilution

1:200

1:500

PROTEIN ARRAY

Antibody HPA048540

Antibody CAB000072

Antibody CAB023409

Antibody CAB026363

Standard validation

Approved

Pass with quality comment low specificity (binding to 1-2 PrESTs >15% and <40%).

Figure description

Antibody specificity analysis with protein arrays. Predicted and matching interactions are shown in green.

Antibody dilution

1:1600

ANTIGEN INFORMATION

Antibody HPA048540

Antibody CAB000072

Antibody CAB023409

Antibody CAB026363

Antigen

Recombinant protein fragment

Not known

Genetic immunization

Length (aa)

Antigen sequence

KSVKKRSVSEIQLMHNLGKHLNSMERVEWLRKKLQDVHNFVALGAPLAPR
DAGSQRPRKKEDNVLVESHEKSLGEADKADVNVLTKAK

Matching transcripts

PTH-001 - ENSP00000282091 [100%]
PTH-002 - ENSP00000433208 [100%]

Other gene match

ANTIGEN VIEW

PTH-001	PTH-002

Contact

contact@proteinatlas.org

The Project

The Human Protein Atlas

The Human Protein Atlas project is funded
by the Knut & Alice Wallenberg foundation.