POC5 - Antibodies - The Human Protein Atlas

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POC5

TISSUE

CELL

CANCER

ANTIBODY INFORMATION

Antibody HPA037509

Antibody HPA037510

Antibody HPA061521

Provider

Atlas Antibodies
Sigma-Aldrich

Product name

HPA037509

HPA037510

HPA061521

Host species

Rabbit

Clonality

pAb

Purity

Affinity purified using the PrEST-antigen as affinity ligand

Other gene match

Released in version

References

VALIDATION SUMMARY

Antibody HPA037509

Antibody HPA037510

Antibody HPA061521

Immunocytochemistry

Immunohistochemistry

Western blot

Protein array

IMMUNOCYTOCHEMISTRY

Antibody HPA037509

Antibody HPA037510

Antibody HPA061521

Formal validation: Independent

Validated

Antibody staining overlaps with antibody HPA061521.

Validated

Antibody staining overlaps with antibody HPA037509.

Standard validation

Uncertain

Figure description

Immunofluorescent staining of human cell line U-251 MG shows localization to nucleoplasm.

Immunofluorescent staining of human cell line RH-30 shows localization to nucleus.

Antibody dilution

1:132

1:200

Literature conformity

The subcellular location is not consistent with literature.

IMMUNOHISTOCHEMISTRY

Antibody HPA037509

Antibody HPA037510

Antibody HPA061521

Standard validation

Approved

Figure description

Immunohistochemical staining of human stomach shows strong cytoplasmic positivity in granular pattern in glandular cells.

Expression

RNA: detected in 35 tissues
Protein: detected in 73 cell types

Retrieval

HIER pH6

Antibody dilution

1:250

Literature conformity

Partly consistent with gene/protein characterization data.

RNA consistency

Mainly not consistent with RNA expression data.

WESTERN BLOT

Antibody HPA037509

Antibody HPA037510

Antibody HPA061521

Standard validation

Supported

Analysis performed using a standard panel of samples.
Band of predicted size in kDa (+/-20%) with additional bands present.

Uncertain

Analysis performed using a standard panel of samples.
Weak band of predicted size but with additional bands of higher intensity also present.

Figure description

Lane 1: Marker [kDa] 230, 130, 95, 72, 56, 36, 28, 17, 11
Lane 2: RT4
Lane 3: U-251 MG
Lane 4: Human Plasma
Lane 5: Liver
Lane 6: Tonsil

Target mass (kDa)

63.4, 60.8

63.4, 60.8, 60.4, 44.3

63.4, 60.8, 60.4

Antibody dilution

1:250

1:690

PROTEIN ARRAY

Antibody HPA037509

Antibody HPA037510

Antibody HPA061521

Standard validation

Approved

Pass with quality comment low specificity (binding to 1-2 PrESTs >15% and <40%).

Supported

Pass with single peak corresponding to interaction only with its own antigen.

Approved

Pass with quality comment low specificity (binding to 1-2 PrESTs >15% and <40%).

Figure description

Antibody specificity analysis with protein arrays. Predicted and matching interactions are shown in green.

Antibody dilution

1:3000

1:8300

ANTIGEN INFORMATION

Antibody HPA037509

Antibody HPA037510

Antibody HPA061521

Antigen

Recombinant protein fragment

Length (aa)

100

Antigen sequence

KWEEYEELLHYAIVTPNIEPCASQSSHPKGELVPDVRISTIHDILHSQGN
NSEVRETAIEVGKGCDFHISSHSKTDESSPVLSPRKPSHPVMD

DVVERACQARAEEVCIQISNDYEAKVAMLSGALENAKAEIQRMQHEKEHF
EDSMKKAFMRGVCALNLEAMTIFQNRNDAGIDSTNNKKEEYGPGVQ

YVPRVVTSAQQKAGRTITARITGRCDFASKNRISSSLAIMGVSPPMSSVV
VEKHHPVTVQTIPQATAAKYPRTIHPESSTSASRSLGTRSAHTQSLTSVH

Matching transcripts

POC5-003 - ENSP00000399481 [100%]
POC5-001 - ENSP00000410216 [98%]

POC5-001 - ENSP00000410216 [100%]
POC5-003 - ENSP00000399481 [100%]
POC5-004 - ENSP00000426796 [100%]
POC5-013 - ENSP00000420971 [100%]

POC5-001 - ENSP00000410216 [100%]
POC5-003 - ENSP00000399481 [100%]
POC5-013 - ENSP00000420971 [100%]

Other gene match

ANTIGEN VIEW

POC5-001	POC5-003	POC5-004	POC5-013

Contact

contact@proteinatlas.org

The Project

The Human Protein Atlas

The Human Protein Atlas project is funded
by the Knut & Alice Wallenberg foundation.