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Immunofluorescent staining of human cell line U-2 OS shows localization to nucleus & microtubules.
Antibody dilution
1:60
Literature conformity
The subcellular location is partly supported by literature or no literature is available.
IMMUNOHISTOCHEMISTRY
Antibody HPA046811
Antibody HPA058495
Standard validation
Supported
Supported
Figure description
Immunohistochemical staining of human fallopian tube shows distinct positivity in cilia.
Immunohistochemical staining of human fallopian tube shows distinct positivity in cytoplasm and cilia in subsets of glandular cells.
Expression
RNA: detected in 8 tissues Protein: detected in 18 cell types
RNA: detected in 8 tissues Protein: detected in 20 cell types
Retrieval
HIER pH6
HIER pH6
Antibody dilution
1:3300
1:350
Literature conformity
No avaliable gene/protein characterization data.
No avaliable gene/protein characterization data.
RNA consistency
Mainly consistent with RNA expression data.
Mainly consistent with RNA expression data.
WESTERN BLOT
Antibody HPA046811
Antibody HPA058495
Standard validation
Uncertain
No bands detected.
Uncertain
Analysis performed using a standard panel of samples. Weak band of predicted size but with additional bands of higher intensity also present.
Figure description
Lane 1: Marker [kDa] 250, 130, 95, 72, 55, 36, 28, 17, 10 Lane 2: Negative control (vector only transfected HEK293T lysate) Lane 3: Over-expression Lysate (Co-expressed with a C-terminal myc-DDK tag (~3.1 kDa) in mammalian HEK293T cells, LY408188)
Target mass (kDa)
24.2, 22, 20.9
24.2, 22, 20.9
Antibody dilution
1:250
1:250
PROTEIN ARRAY
Antibody HPA046811
Antibody HPA058495
Standard validation
Approved
Pass with quality comment low specificity (binding to 1-2 PrESTs >15% and <40%).
Supported
Pass with single peak corresponding to interaction only with its own antigen.
Figure description
Antibody specificity analysis with protein arrays. Predicted and matching interactions are shown in green.
Antibody specificity analysis with protein arrays. Predicted and matching interactions are shown in green.
