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ASAP1

ANTIBODY INFORMATION
 
 

Antibody HPA011136

  

Antibody HPA048565

  

Antibody CAB037292

  

Provider

 Atlas Antibodies
Sigma-Aldrich
   Atlas Antibodies
Sigma-Aldrich
   SIGMA
  

Product name

 HPA011136   HPA048565   A4227  

Host species

 Rabbit   Rabbit   Rabbit  

Clonality

 pAb   pAb   pAb  

Purity

 Affinity purified using the PrEST-antigen as affinity ligand   Affinity purified using the PrEST-antigen as affinity ligand   Protein A/G  

Other gene match

      

Released in version

 14   12   7  

References

      

VALIDATION SUMMARY
 
 

Antibody HPA011136

  

Antibody HPA048565

  

Antibody CAB037292

  
Immunocytochemistry

  Approved    
Immunohistochemistry

Approved   Approved   Approved  
Western blot

Uncertain   Uncertain   Uncertain  
Protein array

Supported   Supported    

IMMUNOCYTOCHEMISTRY
 
 

Antibody HPA011136

  

Antibody HPA048565

  

Antibody CAB037292

  

Standard validation

   Approved    

     

Figure description

   Immunofluorescent staining of human cell line A-431 shows localization to centrosome.    

Antibody dilution

   1:58    

Literature conformity

   The subcellular location is partly supported by literature or no literature is available.    

IMMUNOHISTOCHEMISTRY
 
 

Antibody HPA011136

  

Antibody HPA048565

  

Antibody CAB037292

  

Standard validation

 Approved   Approved   Approved  

     

Figure description

 Immunohistochemical staining of human rectum shows strong cytoplasmic positivity in glandular cells.   Immunohistochemical staining of human bone marrow shows strong cytoplasmic positivity in hematopoietic cells.   Immunohistochemical staining of human colon shows strong cytoplasmic and membrane positivity in glandular cells.  

Expression

 RNA: detected in 37 tissues
Protein: detected in 45 cell types		   RNA: detected in 37 tissues
Protein: detected in 38 cell types		   RNA: detected in 37 tissues
Protein: detected in 76 cell types		  

Retrieval

 HIER pH6   HIER pH6   HIER pH6  

Antibody dilution

 1:35   1:200   1:5000  

Literature conformity

 Consistent with gene/protein characterization data.   Partly consistent with gene/protein characterization data.   Consistent with gene/protein characterization data.  

RNA consistency

 Mainly consistent with RNA expression data.   Mainly consistent with RNA expression data.   Mainly not consistent with RNA expression data.  

WESTERN BLOT
 
 

Antibody HPA011136

  

Antibody HPA048565

  

Antibody CAB037292

  

Standard validation

 Uncertain

Analysis performed using a standard panel of samples.
Only bands not corresponding to the predicted size.		   Uncertain

Analysis performed using a standard panel of samples.
No bands detected.		   Uncertain

Analysis performed using a standard panel of samples.
Only bands not corresponding to the predicted size.		  

     

Figure description

   Lane 1: Marker [kDa] 250, 130, 95, 72, 55, 36, 28, 17, 10
Lane 2: RT4
Lane 3: U-251 MG
Lane 4: Human Plasma
Lane 5: Liver
Lane 6: Tonsil		    

Target mass (kDa)

 52.9   125.5, 124.8, 105.1, 52.9   125.5, 124.8, 105.1, 52.9, 11.2, 4  

Antibody dilution

 1:250   1:290   1:500  

PROTEIN ARRAY
 
 

Antibody HPA011136

  

Antibody HPA048565

  

Antibody CAB037292

  

Standard validation

 Supported

Pass with single peak corresponding to interaction only with its own antigen.		   Supported

Pass with single peak corresponding to interaction only with its own antigen.		    

     

Figure description

 Antibody specificity analysis with protein arrays. Predicted and matching interactions are shown in green.   Antibody specificity analysis with protein arrays. Predicted and matching interactions are shown in green.    

Antibody dilution

 1:3000   1:2350    

ANTIGEN INFORMATION
 
 

Antibody HPA011136

  

Antibody HPA048565

  

Antibody CAB037292

  

Antigen

 Recombinant protein fragment   Recombinant protein fragment   Synthetic peptide  

Length (aa)

 93   122    

Antigen sequence

 
STPRDKQRLSYGAFTNQIFVSTSTDSPTSPTTEAPPLPPRNAGKGNDGGP
SSSSKTTNKFEGLSQQSSTSSAKTALGPRVLPKLPQKVALRKT
  
PKPGELPPKPQLGDLPPKPQLSDLPPKPQMKDLPPKPQLGDLLAKSQTGD
VSPKAQQPSEVTLKSHPLDLSPNVQSRDAIQKQASEDSNDLTPTLPETPV
PLPRKINTGKNKVRRVKTIYDC
  

											 
																				

																		

										
											
Matching transcripts

												
																					ASAP1-015 - ENSP00000429829 [100%] 

											 
																						ASAP1-001 - ENSP00000429900 [100%] 
ASAP1-004 - ENSP00000429391 [100%] 
ASAP1-015 - ENSP00000429829 [100%] 
ASAP1-201 - ENSP00000350297 [100%] 

											 
																						
											 
																				

																		

										
											
Other gene match

												
																					
											 
																						
											 
																						
											 
																				

																		

										ANTIGEN VIEW
									
									
										

		
			

				

										
						

							

								

									
ASAP1-001
ASAP1-004
ASAP1-015
ASAP1-201
								

							

						

							
									

			

				
	

	

		
					
	

													
															
				
						

				 
			

		

			
	











	

		


			

				

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