CD8A - Antibodies - The Human Protein Atlas

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CD8A

TISSUE

CELL

CANCER

ANTIBODY INFORMATION

Antibody HPA037756

Antibody CAB000012

Antibody CAB075722

Provider

Atlas Antibodies
Sigma-Aldrich

DakoCytomation

Atlas Antibodies
Sigma-Aldrich

Product name

HPA037756

M7103

AMAb90883

Host species

Rabbit

Mouse

Clonality

pAb

mAb

Purity

Affinity purified using the PrEST-antigen as affinity ligand

Supernatant

Protein A/G

Other gene match

Released in version

References

VALIDATION SUMMARY

Antibody HPA037756

Antibody CAB000012

Antibody CAB075722

Immunohistochemistry

Western blot

Protein array

IMMUNOHISTOCHEMISTRY

Antibody HPA037756

Antibody CAB000012

Antibody CAB075722

Formal validation: Orthogonal

Validated

Spearman correlation >0.6 for protein and RNA expression in tissues.

Figure description

Distribution of protein expression (antibody staining) and RNA expression (TPM) across 37 tissues.

Standard validation

Supported

Figure description

Immunohistochemical staining of human lymph node shows strong cytoplasmic positivity in subset of non germinal center cells.

Immunohistochemical staining of human lymph node shows strong cytoplasmic positivity in lymphoid cells outside reaction centra.

Immunohistochemical staining of human duodenum shows cytoplasmic positivity in subsets of immune cells

Expression

RNA: detected in 35 tissues
Protein: detected in 15 cell types

RNA: detected in 35 tissues
Protein: detected in 9 cell types

RNA: detected in 35 tissues
Protein: detected in 5 cell types

Retrieval

HIER pH6

HIER pH9

HIER pH6

Antibody dilution

1:800

1:100

1:250

Literature conformity

Consistent with extensive gene/protein characterization data.

RNA consistency

Mainly consistent with RNA expression data.

WESTERN BLOT

Antibody HPA037756

Antibody CAB000012

Antibody CAB075722

Standard validation

Supported

Analysis performed using a standard panel of samples.
Band of predicted size in kDa (+/-20%) with additional bands present.

Uncertain

Analysis performed using a standard panel of samples.
No bands detected.

Uncertain

Analysis performed using a standard panel of samples.
Weak band of predicted size but with additional bands of higher intensity also present.

Figure description

Lane 1: Marker [kDa] 250, 130, 95, 72, 55, 36, 28, 17, 10
Lane 2: RT4
Lane 3: U-251 MG
Lane 4: Human Plasma
Lane 5: Liver
Lane 6: Tonsil

Lane 1: Marker [kDa] 250, 130, 95, 72, 55, 36, 28, 17, 11
Lane 2: RT4
Lane 3: U-251 MG
Lane 4: Human Plasma
Lane 5: Liver
Lane 6: Tonsil

Target mass (kDa)

25.7, 22.1, 21.6

Antibody dilution

1:250

1:500

PROTEIN ARRAY

Antibody HPA037756

Antibody CAB000012

Antibody CAB075722

Standard validation

Approved

Pass with quality comment low specificity (binding to 1-2 PrESTs >15% and <40%).

Figure description

Antibody specificity analysis with protein arrays. Predicted and matching interactions are shown in green.

Antibody dilution

1:12000

ANTIGEN INFORMATION

Antibody HPA037756

Antibody CAB000012

Antibody CAB075722

Antigen

Recombinant protein fragment

Synthetic peptide

Recombinant protein

Length (aa)

Antigen sequence

AASPTFLLYLSQNKPKAAEGLDTQRFSGKRLGDTFVLTLSDFRRENEGYY
FCSALSNSIMYFSHFVPVFLPAKPTTTPAPRPPT

Matching transcripts

CD8A-001 - ENSP00000283635 [100%]
CD8A-002 - ENSP00000321631 [100%]
CD8A-006 - ENSP00000386559 [100%]
CD8A-004 - ENSP00000387314 [85%]

Other gene match

ANTIGEN VIEW

CD8A-001	CD8A-002	CD8A-004	CD8A-006

RELEVANT PUBLICATIONS

Antibody HPA037756

Antibody CAB000012

Antibody CAB075722

The transcriptomic and proteomic landscapes of bone marrow and secondary lymphoid tissues
Andersson S et al
PLoS One 2014;9(12):e115911

Contact

contact@proteinatlas.org

The Project

The Human Protein Atlas

The Human Protein Atlas project is funded
by the Knut & Alice Wallenberg foundation.