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Immunofluorescent staining of human cell line SH-SY5Y shows localization to nucleoplasm.
Antibody dilution
1:200
Literature conformity
The subcellular location is partly supported by literature or no literature is available.
IMMUNOHISTOCHEMISTRY
Antibody HPA034543
Standard validation
Supported
Figure description
Immunohistochemical staining of human testis shows moderate nuclear positivity in cells in seminiferus ducts.
Expression
RNA: detected in 1 tissues Protein: detected in 6 cell types
Retrieval
HIER pH6
Antibody dilution
1:2500
Literature conformity
Consistent with gene/protein characterization data.
RNA consistency
Mainly consistent with RNA expression data.
WESTERN BLOT
Antibody HPA034543
Standard validation
Supported
Band of predicted size in kDa (+/-20%) with additional bands present.
Figure description
Lane 1: Marker [kDa] 250, 130, 95, 72, 55, 36, 28, 17, 10 Lane 2: Negative control (vector only transfected HEK293T lysate) Lane 3: Over-expression Lysate (Co-expressed with a C-terminal myc-DDK tag (~3.1 kDa) in mammalian HEK293T cells, LY408392)
Target mass (kDa)
26.7
Antibody dilution
1:250
PROTEIN ARRAY
Antibody HPA034543
Standard validation
Supported
Pass with single peak corresponding to interaction only with its own antigen.
Figure description
Antibody specificity analysis with protein arrays. Predicted and matching interactions are shown in green.
