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Immunofluorescent staining of human cell line U-2 OS shows localization to nucleoli, nuclear speckles & cytosol.
Antibody dilution
1:52
Literature conformity
The subcellular location is partly supported by literature or no literature is available.
IMMUNOHISTOCHEMISTRY
Antibody HPA044127
Antibody HPA048456
Standard validation
Uncertain
Figure description
Immunohistochemical staining of human lymph node shows strong cytoplasmic positivity in germinal and non-germinal center cells.
Expression
RNA: detected in 36 tissues Protein: detected in 36 cell types
Retrieval
HIER pH6
Antibody dilution
1:150
Literature conformity
Partly consistent with gene/protein characterization data.
RNA consistency
Mainly not consistent with RNA expression data.
WESTERN BLOT
Antibody HPA044127
Antibody HPA048456
Standard validation
Uncertain
Analysis performed using a standard panel of samples. Weak band of predicted size but with additional bands of higher intensity also present.
Uncertain
Analysis performed using a standard panel of samples. Single band larger than predicted size in kDa (+20%) but partly supported by experimental and/or bioinformatic data.
Figure description
Lane 1: Marker [kDa] 250, 130, 95, 72, 55, 36, 28, 17, 10 Lane 2: RT4 Lane 3: U-251 MG Lane 4: Human Plasma Lane 5: Liver Lane 6: Tonsil
Target mass (kDa)
195.3, 118.6, 110.7
195.3, 118.6, 110.7, 32.7
Antibody dilution
1:250
1:260
PROTEIN ARRAY
Antibody HPA044127
Antibody HPA048456
Standard validation
Supported
Pass with single peak corresponding to interaction only with its own antigen.
Supported
Pass with single peak corresponding to interaction only with its own antigen.
Figure description
Antibody specificity analysis with protein arrays. Predicted and matching interactions are shown in green.
Antibody specificity analysis with protein arrays. Predicted and matching interactions are shown in green.
Antibody dilution
1:3000
1:2100
ANTIGEN INFORMATION
Antibody HPA044127
Antibody HPA048456
Antigen
Recombinant protein fragment
Recombinant protein fragment
Length (aa)
102
95
Antigen sequence
VEGGIWSKIFNHKTVVGSKKVNGKIQSTYDQDHTVPAYVPRKLIPITIIK
QVIQNVTHKDSAKSPEKAPLGGVILVHLIIPGLNETTVKLPASLMFEISD
AL
