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Immunofluorescent staining of human cell line A-431 shows localization to cytosol & mitochondria.
Antibody dilution
1:25
Literature conformity
The subcellular location is supported by literature.
IMMUNOHISTOCHEMISTRY
Antibody HPA010537
Standard validation
Uncertain
Figure description
Immunohistochemical staining of human colon shows strong cytoplasmic positivity in glandular cells.
Expression
RNA: detected in 30 tissues Protein: detected in 60 cell types
Retrieval
HIER pH6
Antibody dilution
1:35
Literature conformity
Partly consistent with extensive gene/protein characterization data.
RNA consistency
Mainly not consistent with RNA expression data.
WESTERN BLOT
Antibody HPA010537
Standard validation
Uncertain
Analysis performed using a standard panel of samples. Single band differing more than +/-20% from predicted size in kDa and not supported by experimental and/or bioinformatic data.
Figure description
Lane 1: Marker [kDa] 230, 130, 95, 72, 56, 36, 28, 17, 11 Lane 2: RT4 Lane 3: U-251 MG Lane 4: Human Plasma Lane 5: Liver Lane 6: Tonsil
Target mass (kDa)
225.4
Antibody dilution
1:250
PROTEIN ARRAY
Antibody HPA010537
Standard validation
Approved
Pass with quality comment low specificity (binding to 1-2 PrESTs >15% and <40%).
Figure description
Antibody specificity analysis with protein arrays. Predicted and matching interactions are shown in green.
